通过超瑞利散射测量结合常数：以胆红素与人血清白蛋白的结合为例进行研究

Binding constant measurement by hyper-Rayleigh scattering: bilirubin-human serum albumin binding as a case study.

作者信息

Sri Ranjini Arumugam, Das Puspendu K, Balaram Padmanabhan

机构信息

Department of Inorganic and Physical Chemistry, and Molecular Biophysics Unit, Indian Institute of Science, Bangalore 560 012, India.

出版信息

J Phys Chem B. 2005 Mar 31;109(12):5950-3. doi: 10.1021/jp047268n.

DOI:10.1021/jp047268n

PMID:16851649

Abstract

In this paper, a new application of the hyper-Rayleigh scattering technique in determining multiple binding constants of a small molecule like bilirubin to a macromolecule like the protein human serum albumin has been demonstrated. Human serum albumin has two binding sites for bilirubin, and the binding constants have been measured by carrying out a second harmonic titration of the protein against bilirubin and vice versa. The measured binding constants K(1) = 1.5 +/- 0.43 x 10(7) M(-1) and K(2) = 1.01 +/- 0.16 x 10(6) M(-1) agree well with the reported values obtained by other methods.

摘要

本文展示了超瑞利散射技术在测定小分子（如胆红素）与大分子（如人血清白蛋白）之间多个结合常数方面的新应用。人血清白蛋白有两个胆红素结合位点，通过对蛋白质进行胆红素的二次谐波滴定以及反之亦然的操作来测量结合常数。测得的结合常数K(1) = 1.5 +/- 0.43 x 10(7) M(-1) 和K(2) = 1.01 +/- 0.16 x 10(6) M(-1) 与其他方法得到的报道值吻合良好。