凝胶中二价吸附印迹位点的相关性和交联效应。

Correlation and cross-linking effects in imprinting sites for divalent adsorption in gels.

作者信息

Stancil Kimani A, Feld Michael S, Kardar Mehran

机构信息

Department of Physics, Massachusetts Institute of Technology, Cambridge, Massachusetts 02139, USA.

出版信息

J Phys Chem B. 2005 Apr 14;109(14):6636-9. doi: 10.1021/jp045858f.

DOI:10.1021/jp045858f

PMID:16851745

Abstract

We examine a method to mimic active sites in proteins by chemical imprinting of p-valent templates in heteropolymer gels. Previous studies have confirmed successful formation of sites by adsorption of targets with p >/= 2 contacts. We investigate the recovery of sites with p = 2 imprinted by lead methacrylate Pb(MAAc)(2) (placing two carboxyl groups in close proximity). The improved binding ability of gels with more cross-links, and the relative insensitivity to changes in gel volume contradict simple theory. We conclude that adsorber pairs are predominantly located on the same polymer chain, posing a challenge to mimicking protein-like function.

摘要

我们研究了一种通过在杂聚物凝胶中对p价模板进行化学印迹来模拟蛋白质活性位点的方法。先前的研究已证实，当p≥2个接触点时，通过靶标的吸附成功形成了位点。我们研究了由甲基丙烯酸铅Pb(MAAc)₂印迹的p = 2的位点的恢复情况（将两个羧基紧密放置）。交联更多的凝胶具有更高的结合能力，并且对凝胶体积变化相对不敏感，这与简单理论相矛盾。我们得出结论，吸附剂对主要位于同一聚合物链上，这对模拟蛋白质样功能构成了挑战。