Augood S J, Kiyama H, Faull R L, Emson P C
Department of Neuroendocrinology, A.F.R.C. Institute of Animal Physiology & Genetics Research, Babraham, Cambridge, U.K.
Neuroscience. 1991;44(1):35-44. doi: 10.1016/0306-4522(91)90249-n.
The effect of acute dopamine receptor antagonist treatment on cellular prosomatostatin mRNA expression was investigated in the adult rat striatum using the technique of non-radioactive in situ hybridization. Adult female Wistar rats were given a single intraperitoneal injection of either raclopride (D2 antagonist), SCH 23390 (D1 antagonist) or the D1 (S) enantiomer SCH 23388. Animals were killed either 1, 3 or 9 h following the single i.p. injection and their brains rapidly removed. Striatal sections were then processed for in situ hybridization using an alkaline phosphatase-labelled oligonucleotide probe complementary to a portion of the rat somatostatin cDNA. Blockade of dopamine D1 and D2 receptors resulted in a significant decrease in the cellular content of prosomatostatin mRNA. However, no change in the number of prosomatostatin mRNA containing striatal cells was observed following any of the treatments at any time point. These findings demonstrate that the cellular content of prosomatostatin mRNA in the adult rat striatum is influenced by selective dopamine D1 and D2 receptor antagonists. Further, these findings are consistent with a functional interaction between dopamine and somatostatin in the rat striatum.
采用非放射性原位杂交技术，研究了急性多巴胺受体拮抗剂处理对成年大鼠纹状体中前生长抑素mRNA表达的影响。成年雌性Wistar大鼠单次腹腔注射雷氯必利（D2拮抗剂）、SCH 23390（D1拮抗剂）或D1（S）对映体SCH 23388。单次腹腔注射后1、3或9小时处死动物，并迅速取出其大脑。然后使用与大鼠生长抑素cDNA一部分互补的碱性磷酸酶标记寡核苷酸探针，对纹状体切片进行原位杂交处理。多巴胺D1和D2受体的阻断导致前生长抑素mRNA的细胞含量显著降低。然而，在任何时间点的任何处理后，均未观察到含前生长抑素mRNA的纹状体细胞数量发生变化。这些发现表明，成年大鼠纹状体中前生长抑素mRNA的细胞含量受选择性多巴胺D1和D2受体拮抗剂的影响。此外，这些发现与大鼠纹状体中多巴胺和生长抑素之间的功能相互作用一致。
