Pinna M C, Bauduin P, Touraud D, Monduzzi M, Ninham B W, Kunz W
Dipartimento di Scienze Chimiche, Università di Cagliari-CSGI, Cittadella Monserrato, S.S. 554 Bivio Sestu, 09042 Monserrato-Cagliari, Italy.
J Phys Chem B. 2005 Sep 1;109(34):16511-4. doi: 10.1021/jp051897v.
The effect of choline addition on the salt-induced super activity of horseradish peroxidase (HRP) is investigated. HRP is presented in the literature as an efficient H(2)O(2) scavenger, and choline is the precursor of glycine betaine, a strong osmoprotectant molecule. Both the regulations of H(2)O(2) and of osmoprotectant concentrations are implicated in plants in order to counteract salt-induced cell damage. For the oxidation of 2,2'-azino-bis(3-ethylbenzothiazoline-6-sulfonic acid) diammonium salt (ABTS), sulfate anions were found to play a crucial role in the increase of HRP activity. This induced super activity can be strongly reduced by adding choline chloride. The phenomena provide an example of physicochemical Hofmeister effects playing a central regulatory role in an important biological system.
研究了添加胆碱对盐诱导辣根过氧化物酶（HRP）超活性的影响。文献中表明HRP是一种高效的H₂O₂清除剂，而胆碱是甘氨酸甜菜碱的前体，甘氨酸甜菜碱是一种强大的渗透保护分子。为了对抗盐诱导的细胞损伤，植物中H₂O₂和渗透保护剂浓度的调节都有涉及。对于2,2'-联氮-双-(3-乙基苯并噻唑啉-6-磺酸)二铵盐（ABTS）的氧化，发现硫酸根阴离子在HRP活性增加中起关键作用。添加氯化胆碱可大大降低这种诱导的超活性。这些现象提供了一个物理化学霍夫迈斯特效应在重要生物系统中起核心调节作用的例子。
