脂质微区室中的浓缩DNA。

Condensed DNA in lipid microcompartments.

作者信息

Osfouri Shahriar, Stano Pasquale, Luisi Pier Luigi

机构信息

Institut für Polymere, ETH Zentrum, Universitätstrasse 6, CH-8092 Zurich, Switzerland.

出版信息

J Phys Chem B. 2005 Oct 27;109(42):19929-35. doi: 10.1021/jp053057m.

DOI:10.1021/jp053057m

PMID:16853577

Abstract

DNA was studied in lipid reverse micelles with the aim of investigating the interactions of DNA with lipids in a restricted compartment with minimal water content. Circular dichroic (CD) spectra of DNA at low water content showed the characteristic polymer-salt-induced (psi) spectra of condensed DNA. Dynamic light scattering showed a peak around a radius of 400 nm (corresponding to DNA-containing micelles), and a peak around 2.5 nm (corresponding to "empty" micelles). Fourier Transform-IR (FT-IR) spectroscopy was carried out and analyzed in terms of three distinct states of water inside the micelle water pool, where the local concentration of DNA reached an estimated value of ca. 600 mg/mL, comparable to that found in restricted biological compartments.

摘要

对脂质反胶束中的DNA进行了研究，目的是在水含量最低的受限隔室中研究DNA与脂质的相互作用。低水含量下DNA的圆二色（CD）光谱显示出浓缩DNA的特征性聚合物-盐诱导（psi）光谱。动态光散射显示在约400nm半径处有一个峰（对应于含DNA的胶束），在约2.5nm处有一个峰（对应于“空”胶束）。进行了傅里叶变换红外（FT-IR）光谱分析，并根据胶束水池内水的三种不同状态进行分析，其中DNA的局部浓度达到约600mg/mL的估计值，这与在受限生物隔室中发现的浓度相当。