Pérez S, Miñones J, Espina M, Alsina M A, Haro I, Mestres C
Physicochemistry Department, Faculty of Pharmacy, Av. Joan XXIII s.n. 08028 Barcelona, Spain.
J Phys Chem B. 2005 Oct 27;109(42):19970-9. doi: 10.1021/jp0516240.
Using the Langmuir technique, we have studied the properties at the air/water interface and the interaction of the hepatitis G virus synthetic peptide E1(53-66) and its palmitoyl derivative with membrane phospholipids. These phospholipids had different characteristics referring to the net charge and saturation of the acyl chain. The palmitoyl derivative was more stable at the air/water interface and in the kinetic at constant area measurements showed higher incorporation to the monolayer. The interaction was higher for saturated phospholipids and those with a negative net charge. When the peptides were in the subphase, they produced changes in the miscibility of mixed monolayers composed of DPPC/DPPG or DOPC/DOPG. It can be deduced from the results obtained that electrostatic interactions play a major role, but when the peptide is derivatized with the palmitoyl chain, hydrophobic interactions are added to the former ones. The interaction is also influenced by the saturation of the acyl chain.
利用朗缪尔技术，我们研究了庚型肝炎病毒合成肽E1(53 - 66)及其棕榈酰衍生物在空气/水界面的性质以及它们与膜磷脂的相互作用。这些磷脂在酰基链的净电荷和饱和度方面具有不同的特性。棕榈酰衍生物在空气/水界面更稳定，并且在恒定面积测量的动力学中显示出更高的掺入单层的能力。饱和磷脂和那些具有负净电荷的磷脂之间的相互作用更强。当肽处于亚相时，它们会使由DPPC/DPPG或DOPC/DOPG组成的混合单层的混溶性发生变化。从获得的结果可以推断，静电相互作用起主要作用，但当肽用棕榈酰链衍生化时，疏水相互作用会叠加到前者之上。这种相互作用也受酰基链饱和度的影响。
