Guo Dong-Jie, Xiao Shou-Jun, Xia Bing, Wei Shuai, Pei Jia, Pan Yi, You Xiao-Zeng, Gu Zhong-Ze, Lu Zuhong
State Key Laboratory of Coordination Chemistry, School of Chemistry and Chemical Engineering, Nanjing University, Nanjing 210093, P.R. China.
J Phys Chem B. 2005 Nov 3;109(43):20620-8. doi: 10.1021/jp0535689.
Both end-functionalized (alpha-bromo and omega-carboxy) compounds were first tested for the radical reaction on the silicon-hydride (Si-H) terminated porous silicon (PSi) with/without the presence of diacyl peroxide initiator under microwave irradiation. Then the carboxylic acid monolayers (CAMs) assembled on PSi through the robust Si-C bonds were converted to amino-reactive linker, N-hydroxysuccinimide (NHS)-ester, terminated monolayers. And finally two proteins of bovine serum albumin (BSA) and lysozyme (Lys) were immobilized through amide bonds. The optimum PSi membrane for protein immobilization without collapse, with parameters of porous radii 4-10 nm and depth 0.2-4.6 mum, was prepared from the (100)-oriented p-type silicon wafer. The chemically converted surface products were monitored with Fourier transform infrared spectroscopy (FTIR), X-ray photoelectron spectroscopy (XPS), and field emission scanning electron microscopy (FESEM).
首先，在微波辐射下，对两端功能化的（α-溴和ω-羧基）化合物在有/无二酰基过氧化物引发剂存在的情况下，针对氢化硅（Si-H）封端的多孔硅（PSi）上的自由基反应进行了测试。然后，通过牢固的Si-C键组装在PSi上的羧酸单分子层（CAMs）被转化为氨基反应性连接体、N-羟基琥珀酰亚胺（NHS）酯封端的单分子层。最后，通过酰胺键固定了两种蛋白质，即牛血清白蛋白（BSA）和溶菌酶（Lys）。由（100）取向的p型硅片制备出了用于蛋白质固定且不会塌陷的最佳PSi膜，其多孔半径参数为4 - 10 nm，深度为0.2 - 4.6μm。利用傅里叶变换红外光谱（FTIR）、X射线光电子能谱（XPS）和场发射扫描电子显微镜（FESEM）对化学转化后的表面产物进行了监测。
