甘露醇阴性耐甲氧西林金黄色葡萄球菌的分子鉴定与特征分析
Molecular identification and characterization of mannitol-negative methicillin-resistant Staphylococcus aureus.
作者信息
Shittu Adebayo, Lin Johnson, Morrison Donald
机构信息
School of Biochemistry, Genetics, Microbiology and Plant Pathology, University of KwaZulu-Natal (Westville Campus), Private Bag X54001, Durban, Republic of South Africa.
出版信息
Diagn Microbiol Infect Dis. 2007 Jan;57(1):93-5. doi: 10.1016/j.diagmicrobio.2006.05.004. Epub 2006 Jul 18.
We report on the isolation, molecular identification, and characterization of 5 mannitol-negative methicillin-resistant Staphylococcus aureus (MRSA) from clinical samples in KwaZulu-Natal (KZN) province, South Africa. Identification based on phenotypic testing and polymerase chain reaction detection of the S. aureus species-specific nuc gene and the coagulase gene indicated that the mannitol-negative isolates were S. aureus. Furthermore, they were mecA positive, and SCCmec typing demonstrated that all the isolates harbored type IV SCCmec. API STAPH (Biomerieux, Marcy-l'Etoile, France) misidentified 2 mannitol-negative MRSA that belonged to the major clone in KZN province, as Staphylococcus lugdunensis. Although the prevalence and mechanism of mannitol-negative MRSA is unknown, laboratories are encouraged to investigate S. aureus with atypical characteristics.
我们报告了从南非夸祖鲁-纳塔尔省（KZN）的临床样本中分离、分子鉴定和表征5株甘露醇阴性耐甲氧西林金黄色葡萄球菌（MRSA）的情况。基于表型测试以及对金黄色葡萄球菌种特异性nuc基因和凝固酶基因的聚合酶链反应检测进行的鉴定表明，甘露醇阴性分离株为金黄色葡萄球菌。此外，它们mecA呈阳性，SCCmec分型显示所有分离株均携带IV型SCCmec。API STAPH（法国马西-埃图瓦勒生物梅里埃公司）将2株属于KZN省主要克隆株的甘露醇阴性MRSA误鉴定为路邓葡萄球菌。尽管甘露醇阴性MRSA的流行情况和机制尚不清楚，但鼓励实验室对具有非典型特征的金黄色葡萄球菌进行调查。
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