烟草BY-2细胞中植物发动蛋白的纯化与特性分析
Purification and characterization of plant dynamin from tobacco BY-2 cells.
作者信息
Hamada Takahiro, Igarashi Hisako, Yao Maki, Hashimoto Takashi, Shimmen Teruo, Sonobe Seiji
机构信息
Department of Life Science, Graduate School of Life Science, University of Hyogo, Harima Science Park City, Hyogo, 678-1297 Japan.
出版信息
Plant Cell Physiol. 2006 Aug;47(8):1175-81. doi: 10.1093/pcp/pcj086. Epub 2006 Jul 18.
We purified an 84 kDa polypeptide from the MAP (microtubule-associated protein) fraction of tobacco BY-2 cultured cells. LC/MS/MS (liquid chromatography-tandem mass spectrometry) analysis revealed that this polypeptide is a tobacco homolog of AtDRP3 (Arabidopsis thaliana dynamin-related protein 3). Electron microscopy revealed that NtDRP3 (Nicotiana tabacum dynamin-related protein 3) assembles to form a filamentous structure. When GDP was added to the NtDRP3 fraction, the filaments disappeared and many particles appeared. Biochemical analysis revealed that NtDRP3 could bind to and bundle both microtubules and actin filaments in vitro.
我们从烟草BY-2培养细胞的微管相关蛋白（MAP）组分中纯化出一种84 kDa的多肽。液相色谱-串联质谱（LC/MS/MS）分析表明，该多肽是拟南芥动力蛋白相关蛋白3（AtDRP3）的烟草同源物。电子显微镜观察显示，烟草动力蛋白相关蛋白3（NtDRP3）组装形成丝状结构。当向NtDRP3组分中添加GDP时，丝状结构消失，出现许多颗粒。生化分析表明，NtDRP3在体外可与微管和肌动蛋白丝结合并使其成束。
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