Fujita Yukio, Ohama Eisaku, Takatama Masamitsu, Al-Sarraj Safa, Okamoto Koichi
Department of Neurology, Gunma University Graduate School of Medicine, 3-39-15 Showa-machi, Maebashi, Gunma 371-8511, Japan.
Acta Neuropathol. 2006 Sep;112(3):261-5. doi: 10.1007/s00401-006-0114-4. Epub 2006 Jul 20.
We examined whether the Golgi apparatus (GA) is fragmented in nigral neurons in 18 cases with Parkinson's disease (PD) and in 8 control cases. The nigral neurons in cases with PD showed various degrees of Lewy pathology with alpha-synuclein immunohistochemistry, and we divided the neurons into three subtypes according to differences in alpha-synuclein immunoreactivity: (1) neurons without pale bodies or Lewy bodies, (2) neurons with pale bodies, and (3) neurons with Lewy bodies. In controls, we did not observe fragmented GA in nigral neurons by immunocytochemistry with an anti-TGN46 antibody. In PD, the GA was fragmented in 3% of the nigral neurons without inclusions, and in 5% of the neurons with Lewy bodies. In contrast, fragmented GA was noted in 19% of the neurons containing pale bodies. Since pale bodies represent early stages in the development of brainstem Lewy bodies, our results suggest that the cytotoxicity of alpha-synuclein-positive aggregates is reduced in the process of Lewy body formation.
我们研究了18例帕金森病(PD)患者及8例对照者黑质神经元中的高尔基体(GA)是否破碎。PD患者的黑质神经元经α-突触核蛋白免疫组织化学显示出不同程度的路易病理改变,我们根据α-突触核蛋白免疫反应性的差异将神经元分为三种亚型:(1)无苍白小体或路易小体的神经元;(2)有苍白小体的神经元;(3)有路易小体的神经元。在对照组中,通过抗TGN46抗体免疫细胞化学,我们未在黑质神经元中观察到破碎的GA。在PD中,3%无包涵体的黑质神经元和5%有路易小体的神经元中GA发生了破碎。相比之下,19%含有苍白小体的神经元中观察到GA破碎。由于苍白小体代表脑干路易小体形成的早期阶段,我们的结果表明,在路易小体形成过程中,α-突触核蛋白阳性聚集体的细胞毒性降低。