Gu Li-Hua, Zhang Cheng, Chen Lian-Kang, Zhen Hui-Fen, Cheng Li, Zhou Huai-Gu
Shanghai Institute of Forensic Science, Shanghai, 200083, China.
Fa Yi Xue Za Zhi. 2006 Jun;22(3):196-7, 203.
The STR genotypping of trace oral epithelial cells which are microdissected by laser capture microdissection system (LCM) is explored.
The oral epithelial cells are microdissected using a low-power infrared laser by VERITAS Microdissection Instrument. STR loci of Profiler Plus are detected by multiplex PCR procesures.
DNA genotyping of 7-8 oral epithelial cells are succeeded, and DNA genotyping of 3-4 oral epithelial cells are failed.
It is viable in genotyping of trace oral epithelial cells by Laser Capture Microdissection as a new technology of seperating single cell.
探索用激光捕获显微切割系统（LCM）显微切割微量口腔上皮细胞的STR基因分型。
使用VERITAS显微切割仪通过低功率红外激光对口腔上皮细胞进行显微切割。通过多重PCR程序检测Profiler Plus的STR位点。
成功获得7-8个口腔上皮细胞的DNA基因分型，3-4个口腔上皮细胞的DNA基因分型失败。
激光捕获显微切割作为一种分离单细胞的新技术，用于微量口腔上皮细胞基因分型是可行的。
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