Liu Fang, Wang Jing, Yu Li-juan, Guo Jian-zhang, Gao Jun-wei, Jiao Zhang-ping, Tang Hui
School of Forensic Medicine, Shanxi Medical University, Taiyuan 030001, China.
Fa Yi Xue Za Zhi. 2011 Feb;27(1):33-5, 42.
To assess the application value of laser capture microdissection (LCM) technique for isolating a small number of sperm cells from mixture sample.
Mixture samples were prepared with sperm cells and vaginal epithelia at different concentrations. Both LCM technique and the differential lysis method were employed to obtain sperm cells from the mixture samples, and DNA was extracted by magnetic beads method. STR genotyping was determined using Identifiler kit.
The successful STR genotype rate of sperm cells isolated from mixture samples with LCM technique was 92.86% (13/14). The rate of differential lysis method was 7.14% (1/14). The successful rates between the two methods were statistically different (P < 0.05).
LCM technique can effectively exclude the interference of female cell component and isolate a small number of sperm cells to obtain a single male STR genotyping. LCM technique is obviously better than the differential lysis method.
评估激光捕获显微切割（LCM）技术从混合样本中分离少量精子细胞的应用价值。
制备含有不同浓度精子细胞和阴道上皮细胞的混合样本。采用LCM技术和差异裂解方法从混合样本中获取精子细胞，并通过磁珠法提取DNA。使用Identifiler试剂盒进行STR基因分型。
采用LCM技术从混合样本中分离出的精子细胞，其STR基因分型成功率为92.86%（13/14）。差异裂解方法的成功率为7.14%（1/14）。两种方法的成功率在统计学上存在差异（P < 0.05）。
LCM技术可有效排除女性细胞成分的干扰，分离少量精子细胞以获得单一男性STR基因分型。LCM技术明显优于差异裂解方法。
