Ye Yi-lu, Wang Meng-ling, Chen Li-ping, Liu Lu-ying, Zhang Li-hui, Chen Zhong, Wei Er-qing
Department of Pharmacology, School of Medicine, Zhejiang University, Hangzhou, China.
Yao Xue Xue Bao. 2006 Apr;41(4):333-7.
To determine the effect of histamine on ischemia-induced cellular edema and viability reduction in rat hippocampal slices, and the involved subtypes of histamine receptor in this effect.
In vitro ischemic injury of hippocampal slices was induced by oxygen-glucose deprivation (OGD). The slice injury was determined by real-timely measuring the changes of light transmittance (LT) for the cellular edema in CA1 region of the hippocampal slice, and by detecting the product of 2, 3, 5-triphenyltetrazolium chloride (TTC), formazan, for the slice viability. The effect of histamine at various concentrations on the slice injury was observed, and the blockage by antagonists of histamine receptors was also investigated.
Histamine (0.01-10 micromol x L(-1)) inhibited the peak value of LT during OGD in hippocampal slices and improved the reduced viability after OGD. Diphenhydramine (0.1-10 micromol x L(-1)), an H1 receptor antagonist, did not affect the effect of histamine, while cimetidine (0.1-10 micromol x L(-1)), an H2 receptor antagonist, partly abolished the protective effect of histamine.
Histamine protects hippocampal slices against ischemia-induced cellular edema and viability reduction; this effect might be mediated via, at least partly, H2 receptor.
确定组胺对大鼠海马切片缺血诱导的细胞水肿和活力降低的影响，以及该效应中涉及的组胺受体亚型。
通过氧-葡萄糖剥夺（OGD）诱导海马切片的体外缺血损伤。通过实时测量海马切片CA1区细胞水肿的透光率（LT）变化以及检测2,3,5-三苯基氯化四氮唑（TTC）产物甲臜来确定切片损伤，以评估切片活力。观察不同浓度组胺对切片损伤的影响，并研究组胺受体拮抗剂的阻断作用。
组胺（0.01 - 10 μmol·L⁻¹）抑制了海马切片OGD期间LT的峰值，并改善了OGD后降低的活力。H1受体拮抗剂苯海拉明（0.1 - 10 μmol·L⁻¹）不影响组胺的作用，而H2受体拮抗剂西咪替丁（0.1 - 10 μmol·L⁻¹）部分消除了组胺的保护作用。
组胺可保护海马切片免受缺血诱导的细胞水肿和活力降低；这种效应可能至少部分通过H2受体介导。
