Sámal Filip, Haninec Pavel, Raska Otakar, Dubovỳ Petr
Department of Neurosurgery, 3rd Faculty of Medicine, Charles University, Prague, Czech Republic.
Ann Anat. 2006 Jul;188(4):337-44. doi: 10.1016/j.aanat.2006.01.017.
In view of the Lack of theoretical information, end-to-side neurorrhaphy is a frequent object of experimental interest. End-to-side neurorrhaphy is based on collateral sprouting of an intact axon. The quantitative assessment of collateral sprouts sent by an intact motor and sensory axon was the goal of the present study. End-to-side neurorrhaphy of the distal stump of transected musculocutaneous nerve (MCN) with intact ulnar nerve (UN) was performed in a rat model. Collateral sprouts were quantitatively evaluated by counting of motoneurons and DRG neurons following their retrograde labeling by Fluoro-Ruby and Fluoro-Emerald applied to the UN and MCN, respectively. The results suggest a comparable capacity of both intact sensory and motor axons to send collateral sprouts into a denervated nerve stump. The ratio of sensory/motor neurons, the axons of which reinnervated distal MCN stumps, was very similar to that of intact UN (6.500 and 6.747, respectively), but different from intact MCN (5.029). This suggests that the pruning process occurred to balance the collateral sprouts at a ratio of sensory/motor neurons for the donor UN, but not according to the number of sensory and motor bands of Bungner available in the distal stump of the MCN. The present experimental study confirms end-to-side neurorrhaphy as a suitable method of nerve reconstruction.
鉴于缺乏理论信息，端侧神经缝合术一直是实验研究的热门对象。端侧神经缝合术基于完整轴突的侧支发芽。本研究的目的是对完整运动和感觉轴突发出的侧支芽进行定量评估。在大鼠模型中，将横断的肌皮神经（MCN）远端残端与完整的尺神经（UN）进行端侧神经缝合。分别将荧光红宝石和荧光翡翠应用于尺神经和肌皮神经，通过对运动神经元和背根神经节（DRG）神经元进行逆行标记后计数，对侧支芽进行定量评估。结果表明，完整的感觉和运动轴突向失神经支配的神经残端发出侧支芽的能力相当。重新支配肌皮神经远端残端的感觉/运动神经元轴突比例与完整尺神经的比例非常相似（分别为6.500和6.747），但与完整肌皮神经的比例（5.029）不同。这表明修剪过程发生的目的是使供体尺神经的感觉/运动神经元侧支芽比例达到平衡，而不是根据肌皮神经远端残端中可用的Büngner感觉和运动束的数量。本实验研究证实端侧神经缝合术是一种合适的神经重建方法。
