Matsumoto Yuko, Terui Norifumi, Tanaka Shunitz
Division of Environmental Material Science, Graduate School of Environmental Science, Hokkaido University, Sapporo, Hokkaido, 060-0810, Japan.
Environ Sci Technol. 2006 Jul 1;40(13):4240-4. doi: 10.1021/es060084x.
The surface of a glassy carbon electrode was modified with a DNA-alginate complex film in which water-soluble DNA was encapsulated into a calcium-alginate gel. The resulting modified electrode (DAFE) was used to detect ethidium bromide (EtBr), after its accumulation on the electrode. The intercalative behavior of EtBr for dsDNA in the film was investigated by measuring the electrode response based on the intercalation of EtBr separated from nonspecific interactions (electrostatic interaction). The accumulation of EtBr in the dsDAF was enhanced by applying a negative potential below -200 mV at the dsDAFE. When a positive potential above +700 mV was applied to the dsDAFE for a constant time with stirring in a Tris buffer solution, the amount accumulated decreased. These results indicate that it is possible to electrochemically control the accumulation and release of EtBr when a dsDAFE is used. In addition, the accumulation and detection of EtBr in spiked river water samples and daunomycin, an antitumor agent, is described.
玻碳电极表面用DNA-海藻酸盐复合膜进行了修饰，其中水溶性DNA被包裹在海藻酸钙凝胶中。所得修饰电极（DAFE）用于在溴化乙锭（EtBr）在电极上积累后对其进行检测。通过测量基于与非特异性相互作用（静电相互作用）分离的EtBr嵌入的电极响应，研究了EtBr在膜中对双链DNA的嵌入行为。通过在dsDAFE上施加低于-200 mV的负电位，增强了EtBr在dsDAF中的积累。当在Tris缓冲溶液中搅拌的情况下，在dsDAFE上施加高于+700 mV的正电位一段固定时间时，积累量会减少。这些结果表明，当使用dsDAFE时，可以通过电化学方式控制EtBr的积累和释放。此外，还描述了在加标河水样品中EtBr的积累和检测以及抗肿瘤药物柔红霉素的情况。
