Garbers D L
Howard Hughes Medical Institute, University of Texas Southwestern Medical Center, Dallas 75235.
Can J Physiol Pharmacol. 1991 Oct;69(10):1618-21. doi: 10.1139/y91-239.
Guanylyl cyclase activity exists in the soluble and particulate fractions of most tissue homogenates. Recently, the mRNA encoding members of the enzyme family from both the soluble and particulate fraction have been cloned. The soluble form exists as a heterodimer, both subunits containing cyclase catalytic-like amino acid sequences. Both subunits must be expressed to observe enzyme activity. The particulate (plasma membrane) forms contain a single putative transmembrane domain that separates an extracellular, ligand-binding domain from an intracellular, protein kinase-like and cyclase catalytic region. The protein kinase-like domain appears to serve as an important regulatory element of the receptor.
鸟苷酸环化酶活性存在于大多数组织匀浆的可溶性和颗粒性组分中。最近，编码来自可溶性和颗粒性组分的该酶家族成员的信使核糖核酸已被克隆。可溶性形式以异二聚体存在，两个亚基均含有类似环化酶催化的氨基酸序列。两个亚基都必须表达才能观察到酶活性。颗粒性（质膜）形式含有一个单一的假定跨膜结构域，该结构域将细胞外配体结合结构域与细胞内蛋白激酶样和环化酶催化区域分隔开。蛋白激酶样结构域似乎是受体的重要调节元件。
