Naess-Andresen Carl Fredrik, Walberg Mette, Gaustad Peter
Department of Chemistry, Norwegian University of Life Sciences, Biotechnology and Food Science, Section Chemistry, Aas, Norway.
APMIS. 2006 Jun;114(6):428-35. doi: 10.1111/j.1600-0463.2006.apm_282.x.
The aim of the present study was to test the in vitro antimicrobial properties of a cytotoxic factor isolated from human leukocytes and for an effect on DNA synthesis. A microdilution broth method was used to determine MIC values for fungi and bacteria. Flow cytometry was used to examine the effect on DNA synthesis. The MIC values for fungi were in the range 1-128 microg CF/mL and concentrations 2-4 times the MIC were fungicidal. CF had no effect on bacteria (MIC and MBC values > 1024 microg CF/mL). Growth experiments performed with increasing concentration of CF showed a dose-dependent effect on growth of the yeasts tested. We confirmed that zinc ions could abrogate the antiproliferative effect of CF. Flow cytometric experiments showed that CF had no effect on the cell cyclus. The present study shows that this endogenous substance isolated from human leukocytes has growth inhibitory properties towards fungi. Because of the origin from human leukocytes, CF may have a role in the non-specific defense against fungal infections. The use of CF may potentially have a role in the treatment of infections caused by fungi. Further investigations to characterize CF chemically and to study the antifungal mechanism are necessary.
本研究的目的是测试从人白细胞中分离出的一种细胞毒性因子的体外抗菌特性及其对DNA合成的影响。采用微量稀释肉汤法测定真菌和细菌的最低抑菌浓度（MIC）值。运用流式细胞术检测其对DNA合成的影响。真菌的MIC值范围为1 - 128微克CF/毫升，MIC的2 - 4倍浓度具有杀菌作用。CF对细菌无影响（MIC和MBC值>1024微克CF/毫升）。随着CF浓度增加进行的生长实验表明，其对受试酵母的生长具有剂量依赖性影响。我们证实锌离子可消除CF的抗增殖作用。流式细胞术实验表明CF对细胞周期无影响。本研究表明，从人白细胞中分离出的这种内源性物质对真菌具有生长抑制特性。由于其源自人白细胞，CF可能在针对真菌感染的非特异性防御中发挥作用。CF的使用可能在治疗由真菌引起的感染中具有潜在作用。有必要进一步开展研究以对CF进行化学表征并研究其抗真菌机制。
