Shinozuka Tatsuo, Terada Masaru, Tanaka Einosuke
Department of Central Research Laboratory, School of Medicine, Keio University, 35 Shinanomachi, Tokyo 160-8582, Japan.
Forensic Sci Int. 2006 Oct 16;162(1-3):108-12. doi: 10.1016/j.forsciint.2006.03.038. Epub 2006 Jul 20.
A simultaneous determination of 20 antidepressant drugs (imipramine, amitriptyline, desipramine, trimipramine, nortriptyline, clomipramine, amoxapine, lofepramine, dosulepin, maprotiline, mianserin, setiptiline, trazodone, fluvoxamine, paroxetine, milnacipran, sulpiride, tandspirone, methylphenidate and melitracen) in human plasma was developed using LC/MS with sonic spray ionization (SSI) method. These drugs showed good separation and sensitivity by LC-MS using an Inertsil C-8 column with methanol:10mM ammonium acetate (pH 5.0):acetonitrile (70:20:10) as mobile phase at 0.10 mL/min at 35 degrees C. Solid-phase extraction of these drugs added to the human plasma was performed with an Oasis HLB cartridge column. Recovery and limit of detection of these drugs were between 69 and 102% and between 0.03 and 0.63 microg/mL, respectively. The present procedure offers an easier and more convenient screening method for antidepressants, and will be useful for forensic toxicology investigations.
采用液相色谱-质谱联用（LC/MS）的超声喷雾电离（SSI）方法，建立了同时测定人血浆中20种抗抑郁药物（丙咪嗪、阿米替林、地昔帕明、曲米帕明、去甲替林、氯米帕明、阿莫沙平、洛非帕明、多塞平、马普替林、米安色林、塞替普汀、曲唑酮、氟伏沙明、帕罗西汀、米氮平、舒必利、坦度螺酮、哌甲酯和美利曲辛）的方法。这些药物在35℃下，以甲醇:10mM醋酸铵（pH 5.0）:乙腈（70:20:10）为流动相，流速0.10 mL/min，使用Inertsil C-8柱通过LC-MS显示出良好的分离效果和灵敏度。采用Oasis HLB柱对添加到人体血浆中的这些药物进行固相萃取。这些药物的回收率和检测限分别在69%至102%和0.03至0.63μg/mL之间。本方法为抗抑郁药物提供了一种更简便的筛选方法，将有助于法医毒理学调查。
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