从流式细胞术分选的DNA文库中分离出的新的20号匿名染色体特异性DNA标记的表征与区域定位

Characterization and regional mapping of new anonymous chromosome 20-specific DNA markers isolated from a flow-sorted DNA library.

作者信息

Stolz F M, Pfau H P, Reipen G, Schnittger S, Grzeschik K H, Hansmann I

机构信息

Institut für Humangenetik, Universität Göttingen, Federal Republic of Germany.

出版信息

Genomics. 1991 Dec;11(4):948-55. doi: 10.1016/0888-7543(91)90019-b.

DOI:10.1016/0888-7543(91)90019-b

PMID:1686021

Abstract

Employing the flow-sorted chromosome 20-specific DNA library LL20NS01, we isolated seven novel unique poly- and monomorphic DNA markers specific to human chromosome 20. Initially, 201 phage clones were analyzed regarding insert size and repetitivity. By testing 14 single- and low-copy number clones for their ability to detect RFLPs, three polymorphisms were revealed by two probes, pFMS22-1.4 [D20S22] and pFMS76 [D20S23]. Seven of twenty probes (35%) were assigned to chromosome 20 using a somatic cell hybrid DNA panel. Five of them were regionally mapped by in situ hybridization. Three DNA markers, pFMS51 [D20S29], pFMS76 [D20S23], and pFMS106 [D20S30], were assigned to 20p11.2-p12, and two markers, pFMS22-1.4 [D20S22] and pFMS135 [D20S31], to 20q12-q13.3. Our new chromosome 20-specific DNA markers should be useful for the molecular characterization of this rather underpopulated human chromosome.

摘要

利用流式细胞术分选的20号染色体特异性DNA文库LL20NS01，我们分离出了7个新的、独特的人类20号染色体特异性多态性和单态性DNA标记。最初，对201个噬菌体克隆进行了插入片段大小和重复性分析。通过检测14个单拷贝和低拷贝数克隆检测限制性片段长度多态性（RFLP）的能力，发现两个探针pFMS22 - 1.4 [D20S22]和pFMS76 [D20S23]揭示了3种多态性。使用体细胞杂种DNA面板将20个探针中的7个（35%）定位到20号染色体上。其中5个通过原位杂交进行了区域定位。3个DNA标记pFMS51 [D20S29]、pFMS76 [D20S23]和pFMS106 [D20S30]被定位到20p11.2 - p12，2个标记pFMS22 - 1.4 [D20S22]和pFMS135 [D20S31]被定位到