Yang Fang, He Yuan-li
Department of Obstetrics and Gynecology, Zhujiang Hospital, Southern Medical University, Guangzhou 510282, China.
Nan Fang Yi Ke Da Xue Xue Bao. 2006 Jul;26(7):978-80.
To investigate the effects of human endostatin on proliferation of human umbilical venous endothelial cells, the apoptosis of the endothelial cells treated with recombinant human endostatin was examined by fluorescence method and the cell proliferation activity analyzed by MTT assay. The results showed that human endostatin inhibited the proliferation of the endothelial cells with ED(50) of 550 ng/ml, and the inhibitory effect was enhanced with the increase of endostatin concentration, suggesting a dose-dependent inhibitory effect of human endostatin on endothelial cell proliferation. The mechanism of antiangiogenesis induced by human endostatin is related to the inhibition of cell proliferation and induction of apoptosis of the endothelial cells.
为研究人内皮抑素对人脐静脉内皮细胞增殖的影响，采用荧光法检测重组人内皮抑素处理后内皮细胞的凋亡情况，并用MTT法分析细胞增殖活性。结果显示，人内皮抑素抑制内皮细胞增殖，半数有效剂量（ED50）为550 ng/ml，且随着内皮抑素浓度的增加抑制作用增强，提示人内皮抑素对内皮细胞增殖具有剂量依赖性抑制作用。人内皮抑素诱导抗血管生成的机制与抑制内皮细胞增殖及诱导其凋亡有关。
