• 文献检索
  • 文档翻译
  • 深度研究
  • 学术资讯
  • Suppr Zotero 插件Zotero 插件
  • 邀请有礼
  • 套餐&价格
  • 历史记录
应用&插件
Suppr Zotero 插件Zotero 插件浏览器插件Mac 客户端Windows 客户端微信小程序
定价
高级版会员购买积分包购买API积分包
服务
文献检索文档翻译深度研究API 文档MCP 服务
关于我们
关于 Suppr公司介绍联系我们用户协议隐私条款
关注我们

Suppr 超能文献

核心技术专利:CN118964589B侵权必究
粤ICP备2023148730 号-1Suppr @ 2026

文献检索

告别复杂PubMed语法,用中文像聊天一样搜索,搜遍4000万医学文献。AI智能推荐,让科研检索更轻松。

立即免费搜索

文件翻译

保留排版,准确专业,支持PDF/Word/PPT等文件格式,支持 12+语言互译。

免费翻译文档

深度研究

AI帮你快速写综述,25分钟生成高质量综述,智能提取关键信息,辅助科研写作。

立即免费体验

西班牙旋毛虫分离株中的DNA多态性。

DNA polymorphisms within Spanish Trichinella isolates.

作者信息

Garate T, Albarran E, Bolas-Fernandez F, Martinez-Fernandez A R, Parkhouse R M

机构信息

Division of Immunology, National Institute for Medical Research, London, UK.

出版信息

Parasitol Res. 1991;77(7):602-5. doi: 10.1007/BF00931022.

DOI:10.1007/BF00931022
PMID:1686495
Abstract

A total of 13 Spanish Trichinella isolates were characterised by DNA analysis. Genomic DNA cross-hybridisation tests revealed two distinct groups that exhibited weak cross-reactions. Further diagnostic subdivision was attempted according to restriction-fragment polymorphism using a cloned T. spiralis DNA repetitive fragment as a probe, but no major difference between the two Trichinella groups was observed.

摘要

通过DNA分析对总共13株西班牙旋毛虫分离株进行了特征鉴定。基因组DNA交叉杂交试验显示出两个不同的组,它们表现出微弱的交叉反应。使用克隆的旋毛虫DNA重复片段作为探针,试图根据限制性片段多态性进行进一步的诊断细分,但未观察到这两个旋毛虫组之间有重大差异。

相似文献

1
DNA polymorphisms within Spanish Trichinella isolates.西班牙旋毛虫分离株中的DNA多态性。
Parasitol Res. 1991;77(7):602-5. doi: 10.1007/BF00931022.
2
Repetitive DNA as a tool for the identification and comparison of nematode variants: application to Trichinella isolates.
Mol Biochem Parasitol. 1986 Nov;21(2):113-20. doi: 10.1016/0166-6851(86)90014-9.
3
Trichinella spiralis: genetic evidence for synanthropic subspecies in sylvatic hosts.旋毛虫:野生动物宿主中与人类共生亚种的遗传学证据。
Exp Parasitol. 1987 Oct;64(2):195-203. doi: 10.1016/0014-4894(87)90143-3.
4
Polymerase chain reaction-restriction fragment length polymorphism (PCR-RFLP) for the identification of Trichinella isolates.用于旋毛虫分离株鉴定的聚合酶链反应-限制性片段长度多态性分析(PCR-RFLP)
Parasitology. 1999 Feb;118 ( Pt 2):211-8. doi: 10.1017/s0031182098003679.
5
DNA fingerprints of Trichinella as revealed by restriction fragment length polymorphism and single-strand conformational polymorphism (RFLP-SSCP).通过限制性片段长度多态性和单链构象多态性(RFLP-SSCP)揭示的旋毛虫DNA指纹图谱。
Mol Cell Probes. 2000 Oct;14(5):291-7. doi: 10.1006/mcpr.2000.0316.
6
Molecular characterization of sylvatic isolates of Trichinella spiralis.旋毛虫野生株的分子特征分析
J Parasitol. 1989 Jun;75(3):388-92.
7
Identification of Trichinella isolates by polymerase chain reaction--restriction fragment length polymorphism of the mitochondrial cytochrome c-oxidase subunit I gene.通过聚合酶链反应-线粒体细胞色素c氧化酶亚基I基因的限制性片段长度多态性鉴定旋毛虫分离株。
Int J Parasitol. 1999 Jul;29(7):1113-20. doi: 10.1016/s0020-7519(99)00060-0.
8
Strain-specific 1.7 kilobase repetitive deoxyribonucleic acid sequence family in Trichinella spiralis.
Mol Biochem Parasitol. 1986 Dec;21(3):227-33. doi: 10.1016/0166-6851(86)90128-3.
9
Characterization of the ribosomal DNA from Trichinella spiralis.
Mol Biochem Parasitol. 1989 Jun 1;35(1):67-72. doi: 10.1016/0166-6851(89)90143-6.
10
Restriction fragment length polymorphism (RFLP) analysis of genomic DNA of 5 strains of Trichinella spiralis in China.
Chin Med Sci J. 1995 Sep;10(3):131-5.

本文引用的文献

1
The genome of Schistosoma mansoni: isolation of DNA, its size, bases and repetitive sequences.
Mol Biochem Parasitol. 1982 Aug;6(2):125-37. doi: 10.1016/0166-6851(82)90070-6.
2
Comparative study on polypeptide patterns of larvae of Trichinella isolates by two-dimensional electrophoresis.
J Helminthol. 1987 Sep;61(3):225-8. doi: 10.1017/s0022149x00010051.
3
Trichinella spiralis in an agricultural ecosystem. II. Evidence for natural transmission of Trichinella spiralis spiralis from domestic swine to wildlife.农业生态系统中的旋毛形线虫。II. 旋毛形线虫从家猪自然传播到野生动物的证据。
J Parasitol. 1987 Feb;73(1):103-9.
4
Repetitive DNA as a tool for the identification and comparison of nematode variants: application to Trichinella isolates.
Mol Biochem Parasitol. 1986 Nov;21(2):113-20. doi: 10.1016/0166-6851(86)90014-9.
5
Trichinella spiralis: genetic evidence for synanthropic subspecies in sylvatic hosts.旋毛虫:野生动物宿主中与人类共生亚种的遗传学证据。
Exp Parasitol. 1987 Oct;64(2):195-203. doi: 10.1016/0014-4894(87)90143-3.
6
Molecular approaches to DNA diagnosis.DNA诊断的分子方法。
Parasitology. 1989;99 Suppl:S125-46. doi: 10.1017/s0031182000083463.
7
Molecular characterization of sylvatic isolates of Trichinella spiralis.旋毛虫野生株的分子特征分析
J Parasitol. 1989 Jun;75(3):388-92.
8
Cloning of a species-specific DNA probe from Onchocerca gibsoni.从吉氏盘尾丝虫中克隆物种特异性DNA探针。
Int J Parasitol. 1990 Feb;20(1):31-5. doi: 10.1016/0020-7519(90)90169-n.
9
Detection of specific sequences among DNA fragments separated by gel electrophoresis.在通过凝胶电泳分离的DNA片段中检测特定序列。
J Mol Biol. 1975 Nov 5;98(3):503-17. doi: 10.1016/s0022-2836(75)80083-0.
10
Labeling deoxyribonucleic acid to high specific activity in vitro by nick translation with DNA polymerase I.通过用DNA聚合酶I进行切口平移在体外将脱氧核糖核酸标记至高比活性。
J Mol Biol. 1977 Jun 15;113(1):237-51. doi: 10.1016/0022-2836(77)90052-3.