Dranitsina A S, Telegeev G D, Maliuta S S, Bezrukov V F
National Taras Shevchenko University of Kiev, Faculty of Biology, Kyiv 01033, Prospekt Glushkova 2, Ukraine.
Tsitol Genet. 2006 Mar-Apr;40(2):57-62.
RAPD analysis was used to examine the extent of genetic polymorphism in two populations of Gentoo penguin (Pygoscelis papua) from Antarctic Islands (Petermann and Livingston). The chosen two of three 10 mer oligonucleotide primers accordingly to preliminary results showed different levels of polymorphism in Gentoo penguins at Petermann Island (from 23.53 to 42.86%) and Livingston Island (from 52.94 to 57.14%). Nei's similarity coefficients were in range from 0.5606 (when Gentoo genome profiles were compared with RAPD profiles of two related penguin species: Pygoscelis adeliae (Adelie) and Pygoscelis antarctica (Chinstrep)) to 0.9281 among observed Gentoo penguin populations. Nei's distances values ranged from 0.0746 to 0.5787 among the populations and species. The obtained results will be used for further estimation of genetic diversity of Gentoo penguins and determination of their taxonomic status.
随机扩增多态性DNA分析（RAPD）被用于检测来自南极岛屿（彼得曼岛和利文斯顿岛）的巴布亚企鹅（Pygoscelis papua）两个种群的遗传多态性程度。根据初步结果，从三个10聚体寡核苷酸引物中选择的两个引物，在彼得曼岛的巴布亚企鹅中显示出不同水平的多态性（从23.53%到42.86%），在利文斯顿岛的巴布亚企鹅中也是如此（从52.94%到57.14%）。内氏相似系数范围从0.5606（当将巴布亚企鹅的基因组图谱与两种相关企鹅物种：阿德利企鹅（Pygoscelis adeliae）和南极企鹅（Pygoscelis antarctica）的RAPD图谱进行比较时）到观察到的巴布亚企鹅种群之间的0.9281。种群和物种之间的内氏距离值范围从0.0746到0.5787。所获得的结果将用于进一步评估巴布亚企鹅的遗传多样性及其分类地位的确定。
