Kramer R E, Greiner J W, Colby H D
Steroids. 1975 Jun;25(6):707-16. doi: 10.1016/0039-128x(75)90035-5.
Studies were conducted to further examine the mechanisms responsible for gonadal hormone effects on the rat adrenocortical 11beta-hydroxylase system. Despite higher concentrations of cytochrome P-450 and larger 11-deoxycorticosterone (DOC)-induced difference spectra in adrenal mitochondria from females than males, no sex difference in 11beta-hydroxylase activity was observed. The pregnenolone-induced difference spectrum, indicative of cholesterol binding to cytochrome P-450, also was similar in males and females. Testosterone administration to castrated males lowered both 11beta-hydroxylase activity and mitochondrial cytochrome P-450 content. Estradiol produced the opposite effects in castrated females. However, when given to ACTH-replaced hypophysectomized rats, neither testosterone nor estradiol affected cytochrome P-450 levels or the rate of 11beta-hydroxylation. These observations, taken with the known effects of estradiol and testosterone on ACTH secretion in rats and the effects of ACTH on 11beta-hydroxylation, indicate that gonadal hormone effects on the 11beta-hydroxylase system are mediated by ACTH.
开展了多项研究以进一步探究性腺激素对大鼠肾上腺皮质11β-羟化酶系统产生影响的机制。尽管雌性大鼠肾上腺线粒体中细胞色素P-450的浓度较高，且11-脱氧皮质酮（DOC）诱导的差异光谱比雄性大鼠的更大，但未观察到11β-羟化酶活性存在性别差异。孕烯醇酮诱导的差异光谱（表明胆固醇与细胞色素P-450结合）在雄性和雌性大鼠中也相似。给去势雄性大鼠注射睾酮会降低11β-羟化酶活性和线粒体细胞色素P-450含量。雌二醇对去势雌性大鼠产生相反的作用。然而，当给予促肾上腺皮质激素（ACTH）替代的垂体切除大鼠时，睾酮和雌二醇均不影响细胞色素P-450水平或11β-羟化速率。这些观察结果，结合已知的雌二醇和睾酮对大鼠ACTH分泌的影响以及ACTH对11β-羟化的影响，表明性腺激素对11β-羟化酶系统的影响是由ACTH介导的。
