Pron'ko P S, Gritsenko E A, Ostrovskiĭ Iu M
Vopr Pitan. 1975 May-Jun(3):48-54.
Alimentary B1 avitaminosis was attended by a declining activity of alkaline phosphatase in the brain and spleen of rats. A single administration of oxythiamine to rats in a dose of 400 mg/kg produced during the first hours an increased activity of alkaline phosphatase in the liver, brain and, to a lesser extent, in a number of other organs and reduced the thiamine-diphosphate content in the liver, brain and other tissues. The thiamin-diphosphate level in the brain returned back to normal in 12 hours, and in other tissues-towards the 3--5th day. In 24 hours after introduction of oxythiamine the activity of alkaline phosphatase was up in the liver alone, while in the brain the activity of the enzyme decreased. Thiamine, used in a dose of 400 mg/kg, exerted on the activity of alkaline phosphatase in a number of tissues an action similar to that of oxythiamine. It is suggested that the activation of alkaline phosphatase 3--12 hours following adminstration of a large dose of thiamine or oxythiamine is of a non-specific nature. Subcutaneous introduction of a commercial alkaline phosphatase preparation to rats brought down the thiamine-diphosphate level in all of the tissues under investigation. The presumed mechanisms accounting for the fall of thiamine-diphosphate and the possible part played in this process by alkaline phosphatase are discussed.
大鼠患营养性维生素B1缺乏症时，其脑和脾脏中碱性磷酸酶的活性下降。给大鼠单次注射400mg/kg剂量的氧硫胺，在最初几个小时内，肝脏、脑以及在较小程度上其他一些器官中碱性磷酸酶的活性增加，同时肝脏、脑和其他组织中硫胺二磷酸的含量降低。脑中硫胺二磷酸水平在12小时后恢复正常，而其他组织中的硫胺二磷酸水平在第3至5天恢复正常。注射氧硫胺24小时后，仅肝脏中碱性磷酸酶的活性升高，而脑中该酶的活性降低。400mg/kg剂量的硫胺对许多组织中碱性磷酸酶活性的作用与氧硫胺相似。有人认为，大剂量硫胺或氧硫胺给药后3至12小时碱性磷酸酶的激活具有非特异性。给大鼠皮下注射市售碱性磷酸酶制剂会降低所有被研究组织中的硫胺二磷酸水平。文中讨论了硫胺二磷酸水平下降的推测机制以及碱性磷酸酶在这一过程中可能起的作用。
