[维生素B1缺乏大鼠肝脏透明质浆中游离及结合硫胺素二磷酸的含量]
[Content of free and bound thiamine diphosphate in the liver hyaloplasm of vitamine B1 deficient rats].
作者信息
Ostrovskiĭ Iu M, Voskoboev A I, Gritsenko E A, Grushnik V V
出版信息
Prikl Biokhim Mikrobiol. 1979 Sep-Oct;15(5):728-34.
The amount of free and protein-bound thiamin diphosphate (TDP) in the liver hyaloplasm of B1 vitamin deficient rats has been measured. In the norm the content of protein-bound TDP remains stable (4.5--4.7 micrograms/g tissue) and does not grow upon thiamin injections. The level of the free coenzyme varies appreciably: in the B1-avitaminotic state the content of free TDP decreases, and in the B1-saturated condition it may exceed the norm 4 times. In the liver this enzyme occurs only as a holoenzyme. In case of B1 vitamin deficiency in the diet the transketolase apoform cannot be detected in the liver. A new model for rapid generation of B1-avitaminosis characterized by a significantly lower level of free and bound TDP is described.
已对维生素B1缺乏大鼠肝脏透明质中的游离硫胺素二磷酸（TDP）和与蛋白质结合的TDP含量进行了测定。正常情况下，与蛋白质结合的TDP含量保持稳定（4.5 - 4.7微克/克组织），注射硫胺素后也不会增加。游离辅酶的水平变化明显：在维生素B1缺乏状态下，游离TDP的含量降低，而在维生素B1饱和状态下，其含量可能超过正常水平4倍。在肝脏中，这种酶仅以全酶形式存在。如果饮食中缺乏维生素B1，肝脏中无法检测到转酮醇酶脱辅基形式。本文描述了一种快速产生维生素B1缺乏症的新模型，其特征是游离和结合的TDP水平显著降低。
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