Aquilanti L, Dell'Aquila L, Zannini E, Zocchetti A, Clementi F
Dipartimento di Scienze degli Alimenti, Università Politecnica delle Marche, Ancona, Italy.
Lett Appl Microbiol. 2006 Aug;43(2):161-7. doi: 10.1111/j.1472-765X.2006.01935.x.
Investigation of the autochthonous lactic acid bacteria (LAB) population of the raw milk protected designation of origin Canestrato Pugliese cheese using phenotypic and genotypic methodologies.
Thirty phenotypic assays and three molecular techniques (restriction fragment length polymorphism, partial sequencing of the 16S rRNA gene and recA multiplex PCR assay) were applied to the identification of 304 isolates from raw milk Canestrato Pugliese cheese. As a result, 168 of 207 isolates identified were ascribed to genus Enterococcus, 25 to Lactobacillus, 13 to Lactococcus and one to Leuconostoc. More in details among the lactobacilli, the species Lactobacillus brevis and Lactobacillus plantarum were predominant, including 13 and 10 isolates respectively, whereas among the lactococci, Lactococcus lactis subsp.cremoris [corrected] was the species more frequently detected (seven isolates).
Except for the enterococci, phenotypic tests were not reliable enough for the identification of the isolates, if not combined to the genotype-based molecular techniques. The polyphasic approach utilized allowed 10 different LAB species to be detected; thus suggesting the appreciable LAB diversity of the autochthonous microbial population of the Canestrato Pugliese cheese.
A comprehensive study of the resident raw milk Canestrato Pugliese cheese microbial population has been undertaken.
采用表型和基因型方法研究受原产地保护的普利亚干酪（Canestrato Pugliese cheese）原料乳中的本土乳酸菌（LAB）菌群。
应用30种表型分析方法和3种分子技术（限制性片段长度多态性、16S rRNA基因部分测序和recA多重PCR分析）对普利亚干酪原料乳中的304株分离株进行鉴定。结果显示，在鉴定出的207株分离株中，168株属于肠球菌属，25株属于乳杆菌属，13株属于乳球菌属，1株属于明串珠菌属。更详细地说，在乳杆菌中，短乳杆菌和植物乳杆菌占主导地位，分别有13株和10株分离株；而在乳球菌中，乳酸乳球菌乳脂亚种是最常检测到的菌种（7株分离株）。
除肠球菌外，如果不与基于基因型的分子技术相结合，表型测试对于分离株的鉴定不够可靠。所采用的多相方法能够检测到10种不同的LAB菌种；因此表明普利亚干酪本土微生物菌群中LAB具有明显的多样性。
对普利亚干酪原料乳中的常驻微生物菌群进行了全面研究。
