Guo Zekun, Tang Wenwen, Yuan Jian, Chen Xinya, Wan Bo, Gu Xiuting, Luo Kuntian, Wang Yingli, Yu Long
State Key Laboratory of Genetic Engineering, Institute of Genetics, School of Life Science, Fudan University, Shanghai 200433, PR China.
Biochem Biophys Res Commun. 2006 Sep 8;347(4):867-71. doi: 10.1016/j.bbrc.2006.06.178. Epub 2006 Jul 25.
Brain selective kinase 2 (BRSK2) has been identified as a member of AMPK related kinases. LKB1 can phosphorylate the Thr174 of BRSK2, increasing its activity >50-fold. In this study, we identified cAMP-dependent protein kinase A (PKA) as another upstream kinase of BRSK2, which can phosphorylate BRSK2 at Thr260. The association between these two proteins was confirmed by GST pull-down. Furthermore, our study indicated that the kinase activity of BRSK2 can be increased through phosphorylation by PKA.
脑选择性激酶2（BRSK2）已被鉴定为AMPK相关激酶成员。LKB1可磷酸化BRSK2的苏氨酸174，使其活性增加50倍以上。在本研究中，我们鉴定出环磷酸腺苷依赖性蛋白激酶A（PKA）是BRSK2的另一种上游激酶，其可在苏氨酸260处磷酸化BRSK2。通过谷胱甘肽S-转移酶（GST）下拉实验证实了这两种蛋白之间的关联。此外，我们的研究表明，PKA磷酸化可增加BRSK2的激酶活性。
