Blagburn Byron L, Dryden Michael W, Payne Patricia, Rust Michael K, Jacobs Dennis E, Bond Ross, Hutchinson Melanie J, Denholm Ian, Mehlhorn Heinz, Vaughn Michael, Mencke Norbert, Schroeder Iris, Hostetler Joe, Endrizzi Michael
Department of Pathobiology, College of Veterinary Medicine, Auburn University, Auburn, AL 36849, USA.
Vet Ther. 2006 Summer;7(2):86-98.
A flea larval bioassay was developed by an international team of scientists to monitor the susceptibility of fleas (Ctenocephalides felis) to imidacloprid (Advantage, Bayer HealthCare). The assay was validated using laboratory and field isolates of C. felis. Flea eggs representing different field isolates of C. felis were collected by veterinarians in the United States, United Kingdom, and Germany. Of the 972 flea isolates obtained during the 5-year study, 768 contained sufficient numbers of eggs to conduct the larval bioassay. Greater than 5% survival occurred for only six of the field isolates evaluated. Further evaluation and analysis of these isolates demonstrated that they did not differ significantly in their susceptibility to imidacloprid from the reference strains used to develop the assay. Collections of field flea isolates will continue in an attempt to detect and document any change in the susceptibility of field flea populations to imidacloprid.
一个国际科学家团队开发了一种跳蚤幼虫生物测定法,以监测跳蚤(猫栉首蚤)对吡虫啉(拜耳保健公司的Advantage)的易感性。该测定法使用猫栉首蚤的实验室和野外分离株进行了验证。美国、英国和德国的兽医收集了代表猫栉首蚤不同野外分离株的跳蚤卵。在为期5年的研究中获得的972个跳蚤分离株中,有768个含有足够数量的卵来进行幼虫生物测定。在所评估的野外分离株中,只有6个的存活率超过5%。对这些分离株的进一步评估和分析表明,它们对吡虫啉的易感性与用于开发该测定法的参考菌株没有显著差异。将继续收集野外跳蚤分离株,以试图检测和记录野外跳蚤种群对吡虫啉易感性的任何变化。