Naumova E S, Gazdiev D O, Naumov G I
Mikrobiologiia. 2006 May-Jun;75(3):358-63.
A significant heterogeneity of the species Zygowilliopsis californica was revealed using RFLP-analysis of the PCR-amplified rDNA fragment spanning the 5.8S rRNA gene and the internal transcribed spacers ITS1 and ITS2. Phylogenetic analysis of the nucleotide sequences of ITS1 and ITS2 rDNA differentiated three varieties: Z. californica var. californica, Z. californica var. dimennae, and Z. californica var. fukushimae. The most variable was the ITS 2 region, where 7-26 nucleotide substitutions were revealed. The varieties formed semisterile hybrids with meiotic segregation of control markers. The limits of the phylogenetic species concept are discussed.
利用对跨越5.8S rRNA基因以及内部转录间隔区ITS1和ITS2的PCR扩增rDNA片段进行的限制性片段长度多态性（RFLP）分析，揭示了加利福尼亚合轴威尔酵母（Zygowilliopsis californica）物种的显著异质性。对ITS1和ITS2 rDNA核苷酸序列的系统发育分析区分出了三个变种：加利福尼亚合轴威尔酵母变种加利福尼亚型（Z. californica var. californica）、加利福尼亚合轴威尔酵母变种迪门纳型（Z. californica var. dimennae）和加利福尼亚合轴威尔酵母变种福岛型（Z. californica var. fukushimae）。最具变异性的是ITS2区域，在该区域发现了7 - 26个核苷酸替换。这些变种形成了具有对照标记减数分裂分离的半不育杂种。文中还讨论了系统发育物种概念的局限性。
