Chang H F, Chou C, Yau H F, Chan Y H, Yih J N, Wu J S
Institute of Radiological Sciences, National Yang Ming University, Taipei 112, Taiwan.
J Microsc. 2006 Jul;223(Pt 1):26-32. doi: 10.1111/j.1365-2818.2006.01594.x.
A novel confocal microscope designed for use with turbid media is proposed. We use a Zeeman laser as the light source. Based on the properties of two-frequency polarized photon-pairs and the common-path feature of polarized photon-pairs with heterodyne detection employed in the proposed confocal microscope, three gatings (spatial filtering gating, polarization gating and spatial coherence gating) are thus simultaneously incorporated in the microscope. Experimental results for the angular distribution of polarized photon-pairs in a scattering medium indicate that polarization gating and spatial coherence gating preclude the detection of multiply scattered photons, whereas the pinhole selects the least scattered photon-pairs. Thus, better performance for axial resolution than can be obtained with a conventional confocal microscope is demonstrated experimentally. In addition, the proposed microscope is able to either look deeper into a turbid medium or work with a denser medium; furthermore, the axial resolution is improved.
提出了一种专为与混浊介质配合使用而设计的新型共聚焦显微镜。我们使用塞曼激光器作为光源。基于双频偏振光子对的特性以及所提出的共聚焦显微镜中采用外差检测的偏振光子对的共光路特性,该显微镜同时并入了三种选通技术(空间滤波选通、偏振选通和空间相干选通)。在散射介质中偏振光子对角度分布的实验结果表明,偏振选通和空间相干选通可排除对多次散射光子的检测,而针孔则选择散射最少的光子对。因此,实验证明了该显微镜在轴向分辨率方面比传统共聚焦显微镜具有更好的性能。此外,所提出的显微镜能够更深入地观察混浊介质或在密度更大的介质中工作;而且,轴向分辨率得到了提高。