Li Qing, Nakadai Ari, Matsushima Hiroki, Miyazaki Yoshifumi, Krensky Alan M, Kawada Tomoyuki, Morimoto Kanehisa
Department of Hygiene and Public Health, Nippon Medical School, Tokyo, Japan.
Immunopharmacol Immunotoxicol. 2006;28(2):319-33. doi: 10.1080/08923970600809439.
To explore the effect of forest bathing on the human immune system, we investigated the effect of phytoncides (wood essential oils) on natural killer (NK) activity and the expression of perforin, granzyme A and granulysin in human NK cells. We used NK-92MI cell, an interleukin-2 independent human NK cell line derived from the NK-92 cell, in the present study. NK-92MI cells express the CD56 surface marker, perforin, granzyme A, and granulysin by flow cytometry and are highly cytotoxic to K562 cells in chromium release assay. Phytoncides significantly increase cytolytic activity of NK-92MI cells in a dose-dependent manner and significantly increase the expression of perforin, granzyme A, and granulysin in the NK-92MI cells. Phytoncides also partially, but significantly, restore the decreased human NK activity and the decreased perforin, granzyme A, and granulysin expression in NK-92MI cells induced by dimethyl 2,2-dichlorovinyl phosphate (DDVP), an organophosphorus pesticide. Pretreatment with phytoncides partially prevents DDVP-induced inhibition of NK activity. Taken together, these data indicate that phytoncides significantly enhance human NK activity and this effect is at least partially mediated by induction of intracellular perforin, granzyme A, and granulysin.
为探究森林浴对人体免疫系统的影响，我们研究了植物精气（木材精油）对人自然杀伤（NK）细胞活性以及穿孔素、颗粒酶A和颗粒溶素在人NK细胞中表达的影响。在本研究中，我们使用了NK-92MI细胞，这是一种从NK-92细胞衍生而来的白细胞介素-2非依赖型人NK细胞系。通过流式细胞术检测发现，NK-92MI细胞表达CD56表面标志物、穿孔素、颗粒酶A和颗粒溶素，并且在铬释放试验中对K562细胞具有高度细胞毒性。植物精气以剂量依赖的方式显著增加NK-92MI细胞的细胞溶解活性，并显著增加NK-92MI细胞中穿孔素、颗粒酶A和颗粒溶素的表达。植物精气还能部分但显著地恢复由有机磷农药磷酸二甲基2,2-二氯乙烯酯（DDVP）诱导的NK-92MI细胞中降低的人NK活性以及降低的穿孔素、颗粒酶A和颗粒溶素表达。用植物精气预处理可部分预防DDVP诱导的NK活性抑制。综上所述，这些数据表明植物精气显著增强人NK活性，并且这种作用至少部分是由细胞内穿孔素、颗粒酶A和颗粒溶素的诱导介导的。
