在精子染色质结构分析异常的标本中评估精子核形态。

Evaluation of sperm nuclear morphology in specimens with abnormal sperm chromatin structural assays.

作者信息

Check J H, Summers-Chase D, Swenson K, Check M L, Amadi C

机构信息

The University of Medicine and Dentistry of New Jersey, Robert Wood Johnson Medical School at Camden, Cooper Hospital/University Medical Center, Department of Obstetrics and Gynecology, USA.

出版信息

Arch Androl. 2006 Sep-Oct;52(5):403-6. doi: 10.1080/01485010600692694.

DOI:10.1080/01485010600692694

PMID:16873142

Abstract

Two recent tests have claimed to identify the subfertile male even when other semen parameters were normal: the sperm chromatin structure assay (SCSA) and abnormal sperm nuclear morphology using much higher magnification. The present study attempted to determine if having a high (> 30%) DNA fragmentation index (DFI), thus resulting in an abnormal SCSA test, is associated with a greater likelihood of sperm with abnormal nuclei. Four males with high DFI scores (57.6%, 65.4%, 31.0%, and 35.3%) had their nuclei evaluated by a complex microscope set-up that magnifies the sperm at least 6000x. The corresponding % of normal nuclei was 0%, 20.0%, 23.7% and 40.0%. The mean and median % of normal nuclei was 20.9+/-16.43 and 21.8, respectively. More studies of similarly matched refractory in vitro fertilization cases, where males have normal DFI scores, are needed to determine if having a high DFI index is associated with a lower percentage of normal nuclei.

摘要

最近的两项检测声称，即便其他精液参数正常，也能识别出不育男性：精子染色质结构分析（SCSA）以及使用高得多的放大倍数检测异常精子核形态。本研究试图确定，DNA碎片化指数（DFI）高（>30%）从而导致SCSA检测结果异常，是否与精子核异常的可能性增大有关。对4名DFI得分高（分别为57.6%、65.4%、31.0%和35.3%）的男性，通过一套复杂的显微镜装置评估其精子核，该装置可将精子放大至少6000倍。正常核的相应百分比分别为0%、20.0%、23.7%和40.0%。正常核的平均百分比和中位数百分比分别为20.9±16.43和21.8。需要对更多DFI得分正常的男性不育体外受精病例进行类似匹配研究，以确定高DFI指数是否与正常核百分比降低有关。