Sudhakaran R, Yoganandhan K, Ahmed V P Ishaq, Hameed A S Sahul
Aquaculture Biotechnology Division, Department of Zoology, C. Abdul Hakeem College, Melvisharam-632 509, Vellore Dt., Tamil Nadu, India.
Dis Aquat Organ. 2006 Jun 12;70(1-2):161-6. doi: 10.3354/dao070161.
Five developmental stages of Artemia were exposed to Macrobrachium rosenbergii nodavirus (MrNV) and extra small virus (XSV) by immersion and oral routes in order to investigate the possibility of Artemia acting as a reservoir or carrier of these viruses. The second objective was to determine if virus-exposed Artemia were capable of transmitting the disease to post-larvae (PL) of M. rosenbergii. There was no significant difference in percent mortality between Artemia control groups and groups challenged with these viruses. On the other hand, all the developmental stages of Artemia were positive for both viruses by nested RT-PCR, regardless of the challenge route. In horizontal transmission experiments, 100% mortality was observed in M. rosenbergii PL fed with Artemia nauplii exposed to MrNV and XSV by either challenge route. However, no mortality was observed in PL fed with virus-free Artemia. RT-PCR analysis of the M. rosenbergii PL confirmed the presence of MrNV and XSV in the challenge group and absence in the control group.
卤虫的五个发育阶段通过浸泡和口服途径暴露于罗氏沼虾诺达病毒（MrNV）和超小病毒（XSV），以研究卤虫作为这些病毒的储存宿主或载体的可能性。第二个目标是确定暴露于病毒的卤虫是否能够将疾病传播给罗氏沼虾的后期幼虫（PL）。卤虫对照组与用这些病毒攻击的组之间的死亡率百分比没有显著差异。另一方面，无论攻击途径如何，卤虫的所有发育阶段通过巢式RT-PCR检测这两种病毒均呈阳性。在水平传播实验中，用通过任何一种攻击途径暴露于MrNV和XSV的卤虫无节幼体喂养的罗氏沼虾PL中观察到100%的死亡率。然而，用无病毒卤虫喂养的PL中未观察到死亡。对罗氏沼虾PL的RT-PCR分析证实攻击组中存在MrNV和XSV，而对照组中不存在。
