Guo Yi-qun, Chen Shi-lun
Department of Hematology, Chao Yang Hospital, Capital University of Medical Science, Beijing 100020, China.
Zhonghua Xue Ye Xue Za Zhi. 2006 Apr;27(4):231-4.
To study the alteration and significance of IL-6, vascular endothelial growth factor (VEGF), insulin-like growth factor-1 (IGF-1) in MM progression and the interaction between the three cytokines.
Bone marrow samples from 28 patients with multiple myeloma (MM) (evolution group and steady group), 10 iron deficiency anemia (as normal control) and 2 monoclonal gammopathy of undetermined significance (MGUS) were studied. Bone marrow stromal cells (BMSCs) were established from the bone marrow MNCs. ELISA was performed to detect the concentration of IL-6, VEGF, IGF-1 in culture supernates.
(1) The levels of IL-6 and VEGF secreted by BMSCs were increased in an order from normal control to steady group to evolution group (P < 0.05). However, the concentration of IGF-1 did not increase in MM patients (P > 0.05). (2) The levels of IL-6, VEGF and IGF-1 in the coculture supernates of U266 and BMSCs were increased significantly (P < 0.05), being in an ascending order from normal control to steady group to evolution group (P < 0.05). (3) BMSCs stimulated by exogenous IL-6, VEGF or IGF-1, secreted more VEGF, IGF-1/IL-6, IGF-1/VEGF, IL-6 than unstimulated (P < 0.05). (4) The levels of IL-6, VEGF, IGF-1 secreted by BMSCs from MGUS were similar to that from control group.
IL-6, VEGF, IGF-1 levels associate with evolution of MM; IL-6, VEGF and IGF-1 induce an increase in cytokines secretion of BMSCs.
研究白细胞介素-6（IL-6）、血管内皮生长因子（VEGF）、胰岛素样生长因子-1（IGF-1）在多发性骨髓瘤（MM）进展中的变化及其意义，以及这三种细胞因子之间的相互作用。
研究了28例多发性骨髓瘤患者（进展组和稳定组）、10例缺铁性贫血患者（作为正常对照）和2例意义未明的单克隆丙种球蛋白病（MGUS）患者的骨髓样本。从骨髓单个核细胞中分离培养骨髓基质细胞（BMSCs）。采用酶联免疫吸附测定（ELISA）法检测培养上清液中IL-6、VEGF、IGF-1的浓度。
（1）BMSCs分泌的IL-6和VEGF水平从正常对照到稳定组再到进展组呈递增趋势（P<0.05）。然而，MM患者的IGF-1浓度并未升高（P>0.05）。（2）U266与BMSCs共培养上清液中IL-6、VEGF和IGF-1水平显著升高（P<0.05），从正常对照到稳定组再到进展组呈递增趋势（P<0.05）。（3）外源性IL-6、VEGF或IGF-1刺激的BMSCs分泌的VEGF、IGF-1/IL-6、IGF-1/VEGF、IL-6比未刺激的更多（P<0.05）。（4）MGUS患者BMSCs分泌的IL-6、VEGF、IGF-1水平与对照组相似。
IL-6、VEGF、IGF-1水平与MM的进展相关；IL-6、VEGF和IGF-1可诱导BMSCs细胞因子分泌增加。
