用于生物分子检测的纳米线网络：采用非接触式电阻抗断层成像技术

Nanowires network for biomolecular detection using contactless impedance tomoscopy technique.

作者信息

Gamby J, Abid J-P, Abid M, Ansermet J-P, Girault H H

机构信息

Laboratoire d'Electrochimie Physique et Analytique, ISIC-Station 6, and Laboratoire de Physique des Matériaux Nanostructurés, IPN-Station 3, Ecole Polytechnique Fédérale de Lausanne, CH-1015 Lausanne, Switzerland.

出版信息

Anal Chem. 2006 Aug 1;78(15):5289-95. doi: 10.1021/ac060479z.

DOI:10.1021/ac060479z

PMID:16878861

Abstract

Here we present the detection of ultralow concentrations of biomolecules in a device made from a polycarbonate membrane containing a network of gold nanowires and using a "contactless" impedance tomoscopy technique. The sensor comprises a thin dielectric layer with two parallel band electrodes on the one side and a microchannel containing gold nanowires onto which the adsorption of antibodies occurs. Upon applying a high-frequency ac voltage between the two electrodes, the adsorption process occurring at the surface of the gold nanowires can be followed through contactless impedance measurements. The configuration allows the real-time detection of biomolecules with a bulk concentration in the picomolar range.

摘要

在此，我们展示了在一种由包含金纳米线网络的聚碳酸酯膜制成的装置中，使用“非接触式”阻抗断层扫描技术检测超低浓度生物分子的方法。该传感器包括一侧带有两个平行带状电极的薄介电层以及一个含有金纳米线的微通道，抗体在金纳米线上发生吸附。在两个电极之间施加高频交流电压时，通过非接触式阻抗测量可以跟踪金纳米线表面发生的吸附过程。这种配置能够实时检测浓度在皮摩尔范围内的生物分子。