混合单层保护金纳米团簇作为用于基质辅助激光解吸电离质谱分析的选择性肽提取剂。
Mixed monolayer-protected gold nanoclusters as selective peptide extraction agents for MALDI-MS analysis.
作者信息
Vanderpuije Benjamin N Y, Han Gang, Rotello Vincent M, Vachet Richard W
机构信息
Department of Chemistry, University of Massachusetts, Amherst, Massachusetts 01003, USA.
出版信息
Anal Chem. 2006 Aug 1;78(15):5491-6. doi: 10.1021/ac0604181.
Cationic and anionic nanoparticles selectively target peptides with low and high isoelectric points, respectively. Additionally, their high surface area-to-volume ratios make these nanoparticles (approximately 2-nm core diameter) very efficient extraction and concentration agents. Upon extraction, the peptide-bound nanoparticles can be analyzed by MALDI-MS to provide highly sensitive detection of the targeted peptides. We demonstrate that MALDI-MS can detect peptide concentrations as low as 500 pM from 250-microL solutions using these nanoparticle scaffolds as extraction and concentration agents.
阳离子和阴离子纳米颗粒分别选择性地靶向低等电点和高等电点的肽段。此外，它们高的表面积与体积比使这些纳米颗粒（核心直径约2纳米）成为非常高效的提取和浓缩剂。提取后，与肽结合的纳米颗粒可通过基质辅助激光解吸电离质谱（MALDI-MS）进行分析，以提供对靶向肽段的高灵敏度检测。我们证明，使用这些纳米颗粒支架作为提取和浓缩剂，MALDI-MS能够从250微升溶液中检测低至500皮摩尔的肽浓度。
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