Marsico Alyssa L M, Creran Brian, Duncan Bradley, Elci S Gokhan, Jiang Ying, Onasch Timothy B, Wormhoudt Joda, Rotello Vincent M, Vachet Richard W
Department of Chemistry, University of Massachusetts-Amherst, Amherst, MA, 01003, USA.
Aerodyne Research, Inc., Billerica, MA, USA.
J Am Soc Mass Spectrom. 2015 Nov;26(11):1931-7. doi: 10.1007/s13361-015-1223-x. Epub 2015 Jul 23.
Effective detection of low molecular weight compounds in matrix-assisted laser desorption/ionization (MALDI) mass spectrometry (MS) is often hindered by matrix interferences in the low m/z region of the mass spectrum. Here, we show that monolayer-protected gold nanoparticles (AuNPs) can serve as alternate matrices for the very sensitive detection of low molecular weight compounds such as amino acids. Amino acids can be detected at low fmol levels with minimal interferences by properly choosing the AuNP deposition method, density, size, and monolayer surface chemistry. By inkjet-printing AuNPs at various densities, we find that AuNP clusters are essential for obtaining the greatest sensitivity. Graphical Abstract ᅟ.
在基质辅助激光解吸/电离（MALDI）质谱（MS）中，低分子量化合物的有效检测常常受到质谱低质荷比区域基质干扰的阻碍。在此，我们表明单层保护的金纳米颗粒（AuNP）可作为替代基质，用于非常灵敏地检测低分子量化合物，如氨基酸。通过适当选择AuNP的沉积方法、密度、尺寸和单层表面化学性质，可在低飞摩尔水平检测氨基酸，且干扰极小。通过以各种密度喷墨打印AuNP，我们发现AuNP簇对于获得最高灵敏度至关重要。图形摘要ᅟ。
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