Wang Fengcai, Shang Guangbin, Hao Shui, Jiao Mingda
Institute of Genetics and Cytology, Northeast Normal University, Changchun, China.
Cell Biol Int. 2006 Oct;30(10):836-40. doi: 10.1016/j.cellbi.2006.05.014. Epub 2006 Jun 30.
In order to get a deeper understanding of the relationship between nucleolus structure and its function, the dynamic change and derivation of FC (fibrillar center) and DFC (dense fibrillar component) through interphase were investigated in HeLa cells synchronized at the ultrastructural level. The results showed that there was a process of FC and DFC derivation in the nucleolus of HeLa cells during interphase. In G1 phase there were a few big FCs in the nucleolus of the HeLa cell. In S phase DFC around the FC got thickened and the configuration of the DFC changed. A lot of tiny FCs were derived from parts of the thickened DFC. We called the FC and DFC formed in G1 phase as primary FC (pri-FC) and primary DFC (pri-DFC) and the FC and DFC derived from the thickened pri-DFC as secondary FC (sec-FC) and secondary DFC (sec-DFC). In G2 phase sec-FC and sec-DFC were gradually separated from pri-DFC and scattered evenly in the nucleolus. Few large pri-FCs coexisted with numerous tiny sec-FCs in the nucleolus of HeLa cells in G2 phase. Based on the results of our observation, we suggest here a model of the dynamic change and the process of derivation of FC and DFC through interphase.
为了更深入地了解核仁结构与其功能之间的关系，在超微结构水平上对同步化的HeLa细胞间期核仁中纤维中心（FC）和致密纤维组分（DFC）的动态变化及衍生过程进行了研究。结果表明，HeLa细胞间期核仁中存在FC和DFC的衍生过程。在G1期，HeLa细胞核仁中有一些大的FC。在S期，FC周围的DFC变厚，DFC的形态发生改变。许多微小的FC从增厚的DFC部分衍生而来。我们将G1期形成的FC和DFC称为初级FC（pri - FC）和初级DFC（pri - DFC），将从增厚的pri - DFC衍生而来的FC和DFC称为次级FC（sec - FC）和次级DFC（sec - DFC）。在G2期，sec - FC和sec - DFC逐渐从pri - DFC分离，并均匀地散布在核仁中。在G2期HeLa细胞核仁中，少数大的pri - FC与众多微小的sec - FC共存。基于我们的观察结果，在此提出一个关于FC和DFC在间期的动态变化及衍生过程的模型。
