Iwamoto Jun, Seki Azusa, Takeda Tsuyoshi, Sato Yoshihiro, Yamada Harumoto, Shen Chwan-Li, Yeh James K
Department of Sports Medicine, Keio University School of Medicine, 35 Shinanomachi, Shinjuku-ku, Tokyo 160-8582, Japan.
Exp Anim. 2006 Jul;55(4):349-55. doi: 10.1538/expanim.55.349.
We compared the effects of risedronate (Ris) and calcitriol (Cal) on cancellous osteopenia in rats treated with high-dose glucocorticoid (GC). Forty female Sprague-Dawley rats, 4 months of age, were randomized by the stratified weight method into four groups of 10 rats each according to the following treatment schedule: intact control, and GC administration with vehicle, Ris, or Cal. The GC (methylprednisolone sodium succinate, 5.0 mg/kg, s.c.), Ris (10 microg/kg, s.c.), and Cal (0.1 microg/kg, p.o.) were administered 3 times a week. At the end of the 4-week treatment period, bone histomorphometric analysis was performed for cancellous bone of the proximal tibial metaphysis. The GC administration decreased cancellous bone volume (BV/total tissue volume [TV]), trabecular number (Tb N), and trabecular thickness (Tb Th), as a result of increased bone resorption and decreased bone formation. Ris treatment markedly increased cancellous BV/TV and Tb N above the control level as a result of suppressed bone turnover. On the other hand, Cal treatment attenuated the GC-induced decrease in cancellous BV/TV and Tb Th as a result of suppressed bone resorption and maintained bone formation. This study showed the differential effects of Ris and Cal on cancellous osteopenia in rats treated with high-dose GC.
我们比较了利塞膦酸盐（Ris）和骨化三醇（Cal）对高剂量糖皮质激素（GC）治疗的大鼠松质骨骨质减少的影响。40只4月龄雌性Sprague-Dawley大鼠，根据分层体重法随机分为四组，每组10只，按照以下治疗方案给药：完整对照组，以及给予GC加赋形剂、Ris或Cal。GC（甲泼尼龙琥珀酸钠，5.0mg/kg，皮下注射）、Ris（10μg/kg，皮下注射）和Cal（0.1μg/kg，口服）每周给药3次。在4周治疗期结束时，对胫骨近端干骺端的松质骨进行骨组织形态计量学分析。由于骨吸收增加和骨形成减少，GC给药降低了松质骨体积（BV/总体积[TV]）、骨小梁数量（Tb N）和骨小梁厚度（Tb Th）。Ris治疗由于抑制了骨转换，使松质骨BV/TV和Tb N显著高于对照水平。另一方面，Cal治疗由于抑制了骨吸收并维持了骨形成，减轻了GC诱导的松质骨BV/TV和Tb Th的降低。本研究显示了Ris和Cal对高剂量GC治疗的大鼠松质骨骨质减少的不同作用。
