Zachar V, Ebbesen P
Danish Cancer Society, Department of Virus and Cancer, Aarhus.
Acta Virol. 1991 Sep;35(5):464-8.
Human choriocarcinoma cells of the JAR line, with no demonstrable surface CD4 receptor were infected with human immunodeficiency virus type 1 (HIV-1), strain RF. Primer-directed enzymatic DNA amplification (polymerase chain reaction, PCR) detected the presence of viral DNA when the cultures were investigated at day 5 post-infection (p.i.). The absence of cytopathic changes attributable to virus replication suggested silent infection of these malignant trophoblastic cells. Neither reverse transcriptase (RT) activity nor HIV-specific antigens were found in the culture nutrient medium during JAR cell propagation. However, when the HIV-carrier JAR cells were continuously cultured and the cocultivation was initiated with CEM-SS lymphoblastoid cells after two subsequent passages, rescue of infectious virus was observed.
用人免疫缺陷病毒1型（HIV-1）RF株感染了无明显表面CD4受体的JAR系人绒毛膜癌细胞。在感染后第5天（p.i.）对培养物进行检测时，引物介导的酶促DNA扩增（聚合酶链反应，PCR）检测到了病毒DNA的存在。未观察到由病毒复制引起的细胞病变变化，提示这些恶性滋养层细胞发生了潜伏感染。在JAR细胞增殖过程中，培养营养培养基中未发现逆转录酶（RT）活性和HIV特异性抗原。然而，当持续培养携带HIV的JAR细胞，并在随后传代两次后与CEM-SS淋巴母细胞系进行共培养时，观察到了感染性病毒的释放。
