Methodology of reversible protein labeling for ratiometric fluorescent measurement.

作者信息

Soh Nobuaki, Seto Daisuke, Nakano Koji, Imato Toshihiko

机构信息

Department of Applied Chemistry, Graduate School of Engineering, Kyushu University, 744 Moto-oka, Nishi-ku, Fukuoka 819-0395, Japan.

出版信息

Mol Biosyst. 2006 Feb;2(2):128-31. doi: 10.1039/b515777c. Epub 2005 Dec 16.

DOI:10.1039/b515777c

PMID:16880930

Abstract

The first fluorescent labeling technology, which can induce not only an increase in the fluorescence intensity but also a shift in the fluorescence spectrum, has been developed for "ratiometric" measurements for a protein by utilizing a newly designed "field-sensitive" fluorescent probe and its corresponding unique amino acid tag.

摘要