使用双发射人工分子探针进行标签融合蛋白的比率荧光检测。
Ratiometric fluorescence detection of a tag fused protein using the dual-emission artificial molecular probe.
作者信息
Honda Kei, Nakata Eiji, Ojida Akio, Hamachi Itaru
机构信息
Department of Synthetic Chemistry and Biological Chemistry, Graduate School of Engineering, Kyoto University, Katsura Campus, Nishikyo-ku, Kyoto, 615-8510, Japan.
出版信息
Chem Commun (Camb). 2006 Oct 14(38):4024-6. doi: 10.1039/b608684e. Epub 2006 Aug 17.
DOI:10.1039/b608684e
PMID:17003887
Abstract
We have successfully developed a ratiometric detection system for protein of interest using the complementary recognition pair of the tetra-aspartate peptide tag and the SNARF-appended Zn(ii)-DpaTyr probe.
摘要
我们已成功开发出一种用于检测目标蛋白的比率检测系统，该系统使用了四聚天冬氨酸肽标签与附加了SNARF的锌（II）-二吡啶基酪氨酸探针的互补识别对。
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