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γ-前速激肽原-(72-92)-肽酰胺：一种内源性前速激肽原I基因衍生肽，优先结合神经激肽-2受体。

gamma-Preprotachykinin-(72-92)-peptide amide: an endogenous preprotachykinin I gene-derived peptide that preferentially binds to neurokinin-2 receptors.

作者信息

Dam T V, Takeda Y, Krause J E, Escher E, Quirion R

机构信息

Douglas Hospital Research Centre, McGill University, Verdun, Canada.

出版信息

Proc Natl Acad Sci U S A. 1990 Jan;87(1):246-50. doi: 10.1073/pnas.87.1.246.

DOI:10.1073/pnas.87.1.246
PMID:1688655
原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC53239/
Abstract

The presence of N-terminally extended forms of neurokinin A has recently been reported in the mammalian brain. Among them, gamma-preprotachykinin-(72-92)-peptide amide [gamma-PPT-(72-92)-NH2], a peptide derived by posttranslational processing of gamma-preprotachykinin, is most prominent. We report here that this peptide most likely acts on neurokinin-2 receptor sites since neurokinin A (a putative neurokinin-2 agonist) and gamma-PPT-(72-92)-NH2 are potent competitors of 125I-labeled gamma-PPT-(72-92)-NH2 binding whereas selective neurokinin-1 and -3 agonists are not. Moreover, the distribution of 125I-labeled gamma-PPT-(72-92)-NH2 and 125I-labeled neurokinin A binding sites are very similar in rat brain. On the other hand, 125I-labeled Bolton-Hunter-substance P (a neurokinin-1 ligand) and 125I-labeled Bolton-Hunter-eledoisin (a neurokinin-3 ligand) binding sites are differentially located in this tissue. Thus, it appears that gamma-PPT-(72-92)-NH2 binds to neurokinin-2 receptors and should be considered as a putative endogenous ligand for this receptor class.

摘要

最近有报道称，哺乳动物大脑中存在N端延伸形式的神经激肽A。其中，γ-前速激肽原-(72 - 92)-肽酰胺[γ-PPT-(72 - 92)-NH2]最为突出，它是γ-前速激肽原经翻译后加工衍生而来的一种肽。我们在此报告，该肽很可能作用于神经激肽-2受体位点，因为神经激肽A（一种假定的神经激肽-2激动剂）和γ-PPT-(72 - 92)-NH2是125I标记的γ-PPT-(72 - 92)-NH2结合的有效竞争者，而选择性神经激肽-1和-3激动剂则不是。此外，125I标记的γ-PPT-(72 - 92)-NH2和125I标记的神经激肽A结合位点在大鼠脑中的分布非常相似。另一方面，125I标记的博尔顿-亨特物质P（一种神经激肽-1配体）和125I标记的博尔顿-亨特蛙皮素（一种神经激肽-3配体）结合位点在该组织中的定位不同。因此，γ-PPT-(72 - 92)-NH2似乎与神经激肽-2受体结合，应被视为该受体类别的一种假定内源性配体。

https://cdn.ncbi.nlm.nih.gov/pmc/blobs/1c27/53239/e1713a68eb47/pnas01026-0268-d.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/1c27/53239/e6e3b40d486e/pnas01026-0267-a.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/1c27/53239/3bca6030862c/pnas01026-0267-b.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/1c27/53239/5755cb4ebe1c/pnas01026-0267-c.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/1c27/53239/91123447fb27/pnas01026-0267-d.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/1c27/53239/44a5e5b88e95/pnas01026-0267-e.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/1c27/53239/5ce396cf863f/pnas01026-0267-f.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/1c27/53239/5bcf9248dbe4/pnas01026-0268-a.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/1c27/53239/b37c4bbecdd6/pnas01026-0268-b.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/1c27/53239/b9508301e152/pnas01026-0268-c.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/1c27/53239/e1713a68eb47/pnas01026-0268-d.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/1c27/53239/e6e3b40d486e/pnas01026-0267-a.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/1c27/53239/3bca6030862c/pnas01026-0267-b.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/1c27/53239/5755cb4ebe1c/pnas01026-0267-c.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/1c27/53239/91123447fb27/pnas01026-0267-d.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/1c27/53239/44a5e5b88e95/pnas01026-0267-e.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/1c27/53239/5ce396cf863f/pnas01026-0267-f.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/1c27/53239/5bcf9248dbe4/pnas01026-0268-a.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/1c27/53239/b37c4bbecdd6/pnas01026-0268-b.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/1c27/53239/b9508301e152/pnas01026-0268-c.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/1c27/53239/e1713a68eb47/pnas01026-0268-d.jpg

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