来自铜绿假单胞菌的天青蛋白与细胞色素c-551之间的电子转移。
Electron transfer between azurin and cytochrone c-551 from Pseudomonas aeruginosa.
作者信息
Wilson M T, Greenwood C, Brunori M, Antonini E
出版信息
Biochem J. 1975 Mar;145(3):449-57. doi: 10.1042/bj1450449.
Abstract
The electron-transfer reaction between azurin and cytochrome c1 isolated from Pseudomonas aeruginosa was investigated by rapid-reaction techniques. Temperture-jump studies clearly reveal two chemical relaxations, the amplitudes of which have ikentical spectral distributions, but relaxation times show different dependencies on reactant concentrations. Stopped experiments also showed complex kinetics. A model is proposed which is consistent with the kinetic and equilibrium data obtained. The central feature of this model is the proposal that two intercenvertible forms of reduced azurin exist in solution, only one of which si able to participate directly in the electron-transfer reaction with cytochrome c-551. Support for the hypothesis that two forms of reduced azurin exist is derived from studies on the electron-transfer reaction between azurin and Pseudomonas cytochrome oxidase. The possible physiological significance of such a situation is discussed.
摘要
利用快速反应技术研究了从铜绿假单胞菌中分离出的天青蛋白和细胞色素c1之间的电子转移反应。温度跃升研究清楚地揭示了两种化学弛豫，其幅度具有相同的光谱分布，但弛豫时间对反应物浓度表现出不同的依赖性。停止流动实验也显示出复杂的动力学。提出了一个与所获得的动力学和平衡数据一致的模型。该模型的核心特征是提出溶液中存在两种可相互转化的还原型天青蛋白形式，其中只有一种能够直接与细胞色素c-551参与电子转移反应。关于存在两种还原型天青蛋白形式的假设的支持来自于对天青蛋白和铜绿假单胞菌细胞色素氧化酶之间电子转移反应的研究。讨论了这种情况可能的生理意义。
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THE PURIFICATION AND AMINO ACID COMPOSITION OF PSEUDOMONAS CYTOCHROME C-551.铜绿假单胞菌细胞色素C-551的纯化及氨基酸组成
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Kinetics of electron transfer between azurin and cytochrome 551 from Pseudomonas.绿脓杆菌中蓝铜蛋白与细胞色素551之间的电子转移动力学
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The reaction of Pseudomonas aeurginosa cytochrome c oxidase with sodium metabisulphite.铜绿假单胞菌细胞色素c氧化酶与焦亚硫酸钠的反应
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A temperature-jump study of the reaction between azurin and cytochrome c-551 from Pseudomonas aeruginosa.铜蓝蛋白与铜绿假单胞菌细胞色素c-551之间反应的温度跃升研究。
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