用于确定从血液中分离出的厌氧菌和草绿色链球菌抗生素敏感性的E试验和琼脂稀释法的比较

Comparison of E test and agar dilution methods for determining antibiotic susceptibilities of anaerobic bacteria and viridans streptococci isolated from blood.

作者信息

Hall G, Heimdahl A, Nord C E

机构信息

Department of Oral and Maxillofacial Surgery, Huddinge University Hospital, Karolinska Institute, Stockholm, Sweden.

出版信息

Anaerobe. 1998 Feb;4(1):29-33. doi: 10.1006/anae.1997.0138.

DOI:10.1006/anae.1997.0138

PMID:16887621

Abstract

The antimicrobial susceptibilities of 311 strains of anaerobic bacteria and 140 strains of aerobic bacteria, isolated from blood after dental extraction, were determined by the E test and compared with the results obtained by the agar dilution method on PDM-ASM 2 agar. E test MICs agreed within +/-1 dilution step to the agar dilution MICs in 93%, 52%, 90%, 94% of the tested anaerobes for penicillin V, cefaclor, clindamycin and erythromycin, respectively. For aerobic bacteria the agreement was > or = 90% for the antibiotics tested. The in vitro activities of penicillin V, clindamycin and erythromycin were higher than the activity of cefaclor against the majority of bacteria tested.

摘要

采用E试验法测定了311株从拔牙后血液中分离出的厌氧菌和140株需氧菌的药敏情况，并与在PDM-ASM 2琼脂上用琼脂稀释法得到的结果进行比较。E试验测得的青霉素V、头孢克洛、克林霉素和红霉素的最低抑菌浓度（MIC）与琼脂稀释法测得的MIC在93%、52%、90%、94%的受试厌氧菌中分别在±1个稀释度范围内一致。对于需氧菌，所测抗生素的符合率≥90%。在体外，对于大多数受试细菌，青霉素V、克林霉素和红霉素的活性高于头孢克洛。