Rozkiewicz D, Daniluk T, Sciepuk M, Zaremba M L, Cylwik-Rokicka D, Luczaj-Cepowicz E, Milewska R, Marczuk-Kolada G, Stokowska W
Department of Pediatric Infectious Diseases, Medical University of Białystok, Poland.
Adv Med Sci. 2006;51 Suppl 1:191-5.
The aim of this study was to evaluated the prevalence rate of oral viridans group streptococci (VGS) and their susceptibilities to some antibiotics in healthy children.
Samples of pharyngeal swabs and supragingival dental plaques for microbiological studies were collected from 206 healthy children, aged 4-18 years. Additionally, 75 samples of carious lesions from children with dental caries were included. The streptococci were isolated and identified using standard methods and commercial identification kits. For performance of antibacterial susceptibility testing of VGS strains disk diffusion and/or breakpoints procedures were used according to NCCLS standards and criteria. A total of 425 VGS strains were tested against penicillin, ampicillin, erythromycin, clindamycin, tetracycline, doxycycline, ciprofloxacin and vancomycin.
A total of 239 VGS strains belonging to 8 species from pharyngeal swabs of 192 (93.2%) children were isolated. VGS strains from supragingival plaques were isolated in 149 (72.3%) healthy children (p < 0.05), and from carious lesions in 37 (49.3%) children with dental caries. VGS strains of S. mitis species were isolated most frequently from 4-5 year old as compared to 12 and 18 year old children (p < 0.05), while S. vestibularis strains isolated most often in 12 year old ones (p < 0.05). Among 425 VGS strains, high level of penicillin resistance (MIC > or = 2.0 mg/L) was shown in 71 (16.7%) strains, 33 (46.5%) of them belonged to S. mitis species. VGS strains were also resistant to erythromycin (23.5%), clindamycin (23.1%), tetracyclines (T-52%, DOX-16%), gentamycin (25.9%) and ciprofloxacin (55.2%). All VGS strains were vancomycin - susceptible.
本研究旨在评估健康儿童口腔甲型溶血性链球菌（VGS）的流行率及其对某些抗生素的敏感性。
从206名4至18岁的健康儿童中采集咽拭子和龈上牙菌斑样本用于微生物学研究。此外，还纳入了75名患有龋齿儿童的龋损样本。采用标准方法和商业鉴定试剂盒分离并鉴定链球菌。根据美国国家临床实验室标准委员会（NCCLS）的标准和准则，使用纸片扩散法和/或断点法对VGS菌株进行抗菌药敏试验。共对425株VGS菌株进行了青霉素、氨苄西林、红霉素、克林霉素、四环素、多西环素、环丙沙星和万古霉素的药敏测试。
从192名（93.2%）儿童的咽拭子中分离出总共239株属于8个菌种的VGS菌株。在149名（72.3%）健康儿童的龈上菌斑中分离出VGS菌株（p < 0.05），在37名（49.3%）患有龋齿儿童的龋损中分离出VGS菌株。与12岁和18岁儿童相比，4至5岁儿童中最常分离出缓症链球菌菌种的VGS菌株（p < 0.05），而前庭链球菌菌株最常分离自12岁儿童（p < 0.05）。在425株VGS菌株中，71株（16.7%）表现出高水平的青霉素耐药性（MIC≥2.0 mg/L），其中33株（46.5%）属于缓症链球菌菌种。VGS菌株还对红霉素（23.5%）、克林霉素（23.1%）、四环素（T - 52%，DOX - 16%）、庆大霉素（25.9%）和环丙沙星（55.2%）耐药。所有VGS菌株对万古霉素敏感。
