Tabibzadeh S
Department of Pathology, City Hospital Center, Elmhurst, New York 11373.
J Clin Endocrinol Metab. 1990 Feb;70(2):437-43. doi: 10.1210/jcem-70-2-437.
Human endometrium harbors a major population of lymphoid cells. The proliferation of these cells is assessed by the in situ labeling of endometrial sections for Ki67 (a proliferation marker) as well as in vitro incorporation of bromodeoxyuridine (BrdU; a thymidine analog) into S-phase cells in vibratome sections of endometria. Using the avidin-biotinperoxidase complex method, sections of endometria and vibratome sections of endometria are labeled for Ki67 and BrdU, respectively. Subsequently, they are immunostained for CD45 (a lymphoid cell marker), CD3 (a T cell marker), and CD11c (a macrophage marker) molecules. Lymphoid cells scattered or aggregated in the endometrial stroma as well as intraglandular lymphoid cells exhibit Ki67 positivity throughout the menstrual cycle. The proliferative activity of the lymphoid cells, including the CD45, CD3, and CD11c positive cells scattered in the stroma, is markedly increased in the secretory phase. Similar, however less conspicuous, increased Ki67 labeling is observed in the lymphoid cells within lymphoid aggregates. The increased proliferative activity of the lymphoid cells in the secretory phase is confirmed by BrdU labeling in the vibratome sections. In the proliferative phase the non-CD45-positive cells in the endometrial stroma exhibit low proliferative activity, and in the secretory phase, Ki67 labeling in the endometrial stroma is primarily confined to the CD45-positive cells. The findings suggest that in situ proliferation is one mechanism by which the endometrial lymphoid cell pool within endometrium is restored after each menstrual shedding.
人类子宫内膜中存在大量淋巴细胞。这些细胞的增殖情况通过对子宫内膜切片进行Ki67（一种增殖标志物）的原位标记以及体外将溴脱氧尿苷（BrdU；一种胸苷类似物）掺入子宫内膜振动切片中的S期细胞来评估。采用抗生物素蛋白-生物素-过氧化物酶复合物法，分别对子宫内膜切片和子宫内膜振动切片进行Ki67和BrdU标记。随后，对它们进行CD45（一种淋巴细胞标志物）、CD3（一种T细胞标志物）和CD11c（一种巨噬细胞标志物）分子的免疫染色。在整个月经周期中，散在或聚集于子宫内膜基质中的淋巴细胞以及腺内淋巴细胞均表现出Ki67阳性。包括散在基质中的CD45、CD3和CD11c阳性细胞在内的淋巴细胞的增殖活性在分泌期显著增加。在淋巴细胞聚集区内的淋巴细胞中也观察到类似但不太明显的Ki67标记增加。通过对振动切片进行BrdU标记证实了分泌期淋巴细胞增殖活性的增加。在增殖期，子宫内膜基质中的非CD45阳性细胞增殖活性较低，而在分泌期，子宫内膜基质中的Ki67标记主要局限于CD45阳性细胞。这些发现表明，原位增殖是月经脱落后子宫内膜内淋巴细胞池得以恢复的一种机制。
