Vianello Marika, Giometto Bruno, Vassanelli Stefano, Canato Marta, Betterle Corrado, Mucignat Carla
Department of Human Anatomy and Physiology, Section of Physiology, Via Marzolo 3, 35131 Padova, Italy.
Exp Neurol. 2006 Dec;202(2):514-8. doi: 10.1016/j.expneurol.2006.06.012. Epub 2006 Aug 7.
Immunological derangement is assumed to be present in a subgroup of patients affected by drug-resistant epilepsy with serum harboring anti-glutamic acid decarboxylase autoantibodies (GAD-Ab). To further investigate the specific reactivity of GAD-Ab with target cells, we tested sera from drug-resistant epileptics harboring GAD-Ab on cultured fetal rat hippocampal neurons. As a control, we tested sera from GAD-Ab-negative epileptics and GAD-Ab-positive patients affected by Stiff Person Syndrome (SPS), ataxia or diabetes. A specific pattern of reactivity, varying according to disease, was detected on application of sera from GAD-Ab-positive patients with epilepsy, SPS and ataxia, but no specific labeling was found on application of sera from patients with GAD-Ab-negative epilepsy or from GAD-Ab-positive diabetic controls.
免疫紊乱被认为存在于一部分耐药性癫痫患者中，这些患者血清中含有抗谷氨酸脱羧酶自身抗体（GAD-Ab）。为了进一步研究GAD-Ab与靶细胞的特异性反应性，我们用携带GAD-Ab的耐药性癫痫患者的血清检测培养的胎鼠海马神经元。作为对照，我们检测了GAD-Ab阴性癫痫患者以及患有僵人综合征（SPS）、共济失调或糖尿病的GAD-Ab阳性患者的血清。在应用来自癫痫、SPS和共济失调的GAD-Ab阳性患者的血清时，检测到了根据疾病而变化的特异性反应模式，但在应用来自GAD-Ab阴性癫痫患者或GAD-Ab阳性糖尿病对照患者的血清时未发现特异性标记。
