已建立的肾细胞系LLC-PK中多胺的转运系统。一种钠依赖性转运体的极化表达。
Transport systems for polyamines in the established renal cell line LLC-PK. Polarized expression of an Na(+)-dependent transporter.
作者信息
Van Den Bosch L, De Smedt H, Missiaen L, Parys J B, Borghgraef R
机构信息
Physiological Laboratory, Katholicke Universiteit Leuven, Belgium.
出版信息
Biochem J. 1990 Jan 15;265(2):609-12. doi: 10.1042/bj2650609.
Abstract
We present evidence for the existence of an Na(+)-dependent transporter and an Na(+)-independent transporter for polyamines in LLC-PK1 cells. Both transporters could be discriminated by their sensitivity to inhibitors, particularly rho-chloromercuriphenyl sulphate and various polycationic molecules. By using cell monolayers grown on a permeable filter support, we have found that the Na(+)-dependent polyamine uptake occurred preferentially from the basolateral side. The Na(+)-independent uptake, on the other hand, occurred to the same extent from either the apical or the basolateral side.
摘要
我们提供了LLC - PK1细胞中存在多胺的钠依赖性转运体和钠非依赖性转运体的证据。两种转运体可通过它们对抑制剂的敏感性来区分，尤其是对氯汞苯磺酸和各种聚阳离子分子的敏感性。通过使用生长在可渗透滤膜支架上的细胞单层，我们发现钠依赖性多胺摄取优先从基底外侧发生。另一方面，钠非依赖性摄取从顶端或基底外侧发生的程度相同。
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本文引用的文献
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Protein measurement with the Folin phenol reagent.使用福林酚试剂进行蛋白质测定。
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Localization of the Na+-sugar cotransport system in a kidney epithelial cell line (LLC PK1).钠糖共转运系统在肾上皮细胞系（LLC PK1）中的定位。
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Intracellular putrescine and spermidine deprivation induces increased uptake of the natural polyamines and methylglyoxal bis(guanylhydrazone).细胞内腐胺和亚精胺缺乏会导致天然多胺和甲基乙二醛双（脒腙）的摄取增加。
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