铜盐、镍盐及金属 - 促性腺激素释放激素复合物在体外对促黄体生成素的释放作用
LH release by Cu and Ni salts and metal-GnRH complexes, in vitro.
作者信息
Michaluk Anna, Blitek Agnieszka, Gajewska Alina, Kaczmarek Monika M, Gromadzka-Hliwa Katarzyna, Kochman Helena, Ziecik Adam J, Kochman Kazimierz
机构信息
The Kielanowski Institute of Animal Physiology and Nutrition, Polish Academy of Sciences, 05-110 Jabłonna near Warsaw, Poland.
出版信息
Neuro Endocrinol Lett. 2006 Aug;27(4):483-6.
The present studies were undertaken to examine the effect of copper and nickel salts and their complexes with GnRH on LH release from the pig anterior pituitary cells in vitro. The potency of Cu-GnRH and Ni-GnRH binding to GnRH receptors with iodinated GnRH as a radioactive tracer was also verified. The incubation of pig pituitary cells with Cu and Ni acetate salts showed no effect of the studied ions on LH release at any concentration used. However, nickel salt at a lower dose (10(-10) and 10(-9) M) tended to decrease LH output. By contrast, the native GnRH as well as its metal complexes significantly stimulated LH release after three hours of treatment and Cu-GnRH was found to be the most effective. The results showed that Cu and Ni complexes with GnRH but not their acetate salts are effective in LH release from pig pituitary cells collected from adult female pigs.
本研究旨在检测铜盐和镍盐及其与促性腺激素释放激素（GnRH）的复合物对体外培养的猪垂体前叶细胞促黄体生成素（LH）释放的影响。还以碘化GnRH作为放射性示踪剂，验证了铜-GnRH和镍-GnRH与GnRH受体结合的能力。用醋酸铜盐和镍盐孵育猪垂体细胞，在所使用的任何浓度下，所研究的离子对LH释放均无影响。然而，较低剂量（10⁻¹⁰和10⁻⁹ M）的镍盐倾向于降低LH产量。相比之下，天然GnRH及其金属复合物在处理三小时后显著刺激了LH释放，并且发现铜-GnRH最为有效。结果表明，与GnRH形成的铜和镍复合物而非它们的醋酸盐，对从成年母猪采集的猪垂体细胞释放LH有效。
Zotero 插件